Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit
Table 1
EST databases used for unitag annotations from sugarcane HT-SuperSAGE libraries (tolerant/sensitive genotypes after 24 h of continuous dehydration or regular daily irrigation) and BLASTn nonredundant results.
Species
Database
Annot.*/ESTs
PIP
TIP
NIP
SIP
Total BlastN Align.
Score 52
Align. (up to one mismatch)
Unitags
Unique ESTs+
Unitags with GO1
Saccharum spp.
dbEST
24/256,636
0
0
0
0
154
41
105
43
34
23
Saccharum officinarum
SoGI
265/121,342
157
85
21
11
683
158
425
260
127
50
Sorghum bicolor
SbGI
68/46,043
34
23
5
6
74
11
63
19
7
74
Zea mays
ZmGI
347/315,134
142
154
35
19
49
7
29
13
15
20
Oryza sativa
OsGI
283/201,220
121
95
33
7
119
26
72
20
86
3
Panicum virgatum
PaviGI
147/85,244
58
45
28
6
174
31
116
22
57
0
Triticum aestivum
TaGI
542/222,152
138
128
32
14
253
20
197
23
123
5
Hordeum vulgare
HvGI
110/83,101
32
33
12
9
34
2
30
16
21
7
Festuca arundinacea
FaGI
27/30,244
5
1
1
1
39
10
27
17
14
5
Total
1,913/1,361,144
687
564
167
73
1,579
306
1,064
289#
484
45#
Aquaporin, tonoplast intrinsic protein and major intrinsic protein, membrane integral protein (PIP, TIP, NIP e SIP); +number of nonredundant ESTs (putative aquaporins) anchoring unitags, 1“water transporter”; #number without redundancy among the nine databanks.
