Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit

Table 5

Primers sequences for RT-qPCR of SsPIP1-1, SoPIP1-3/PIP1-4 (designed from sugarcane ESTs), 25S rRNA, and GAPDH (as reference genes).

Unitag	EST/cluster	Database*	Gene	Primers	Tm (°C)	Amplicon (bp)	Regression coefficient (𝑅^2)	Amplification efficiency (%)
SD264077	gi∣35203438	dbEST	SsPIP1-1	5′-GTTCCTATCCTTGCCCCACT-3′; 3′-AGGCGTGATCCCTGTTGTAG-5′	84.6	134	0.995	95.55
SD231548	TC127588	SoGI	SoPIP1-3/PIP1-4	5′-GACTCCCATGTTCCTATCCTTG-3′; 3′-CGTGATCCCTGTTGTAGATGAT-5′	84.3	142	0.992	93.47
—	gi∣33464288	dbEST	25S rRNA	5′-GCAGCCAAGCGTTCATAG-3′; 3′- CGGCACGGTCATCAGTAG-5′	82.9	172	0.999	99.82
—	TC531505	SoGI	GAPDH	5′-GGTTCACTTGAAGGGTGGTG-3′; 3′- TGAGGTGTACCTGTCCTCGTT-5′	81.8	100	0.984	100.89

Isoforms are preceded by the abbreviated species name (So: Saccharum officinarum; Ss: Saccharum spp.).
∗Databases: dbEST (NCBI; http://www.ncbi.nlm.nih.gov/), Gene Index (SoGI; http://compbio.dfci.harvard.edu/tgi/)].