Journal of Nucleic Acids

Research Article

Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit

Table 6

Relative expression rates of aquaporins PIP1-1 (SD264077 unitag) and PIP1-3/PIP1-4 (SD231548 unitag) in bulks of tolerant or sensitive genotypes, respectively, and RT-qPCR data (both in bulks and each genotype).


Technique and genotypes	Target gene
	PIP1-1	PIP1-3/PIP1-4
	Modulation of gene expression^&

HT-SuperSAGE
Tolerant bulk¹	3.580*/UR	3.180*/UR
Sensitive bulk¹	−4.560*/DR	1.280*/ns
RT-qPCR and Tolerant Genotypes
CTC6	1.652^#/UR	1.271^#/ns
CTC15	0.740^#/DR	0.670^#/DR
SP83-2847	1.836^#/UR	1.468^#/UR
SP83-5073	0.324^#/DR	0.383^#/DR
RT-qPCR and sensitive Genotypes
CTC9	1.030^#/ns	1.205^#/ns
CTC13	0.635^#/DR	0.644^#/DR
SP90-1638	1.536^#/ns	1.236^#/ns
SP90-3414	0.324^#/DR	0.383^#/DR

Bulk of the four tolerant or sensitive genotypes; ^&P < 0.05;*fold change [FC: ratio of the frequencies (normalized to 1,000,000) observed in the stressed library in relation to the control library]; ^#relative expression level using the REST software (v. 2.0.13) [26]; DR: down-regulated; UR: up-regulated; ns: not significant at P < 0.05.