Increasing Membrane Cholesterol Level Increases the Amyloidogenic Peptide by Enhancing the Expression of Phospholipase C
Figure 5
The effect of augmentation of membrane cholesterol levels on PLCβ1 and PLCβ3 expressions were blocked by the presence of antisense oligonucleotides. (a, b) The effect of cholesterol on PLCβ1 and PLCβ3 expressions were prevented by the presence of antisense oligonucleotides. APP-transfected HeLa cells were incubated with or without 75 μM water-soluble cholesterol for 2 h, and the expression levels of PLCβ1 and PLCβ3 were tested using western blotting. In some cells, 10 μM antisense oligonucleotides directed against PLCβ1 (anti β1) or PLCβ3 (anti β3) were pretreated for 4 h before the cholesterol treatment. Similar results were obtained from 4 different experiments. Antisense oligonucleotides having no specific target were used for controls in all of experiments. (c, d) Bars correspond to the densitometric analysis of the expression levels of PLCβ1 and PLCβ3, respectively (). *.
