Simple Repeat-Primed PCR Analysis of the Myotonic Dystrophy Type 1 Gene in a Clinical Diagnostics Environment

Figure 2

Agarose gel electrophoresis of PCR products for each of four amplifications of the CTG repeat region of the DMPK gene. (a) Primers P1-FAM and P2; (b) primers P1-FAM and P4CTG (TP-PCR forward primer combination) with P3 primer; (c) TP-PCR forward primer combination; (d) P2-FAM and P4CAG (TP-PCR reverse primer combination). From left to right the gels show DNAs with repeat lengths of [7,11], [11,13], [12,13], [5,14], [11,15], [11,26], [5,27], [13,54], [5,74], [4,77], [11,84], [5,120], [5,250], [5,380], [12,500], [14,530], [12,700], [1000--1500], [1067--1600], [5,405], and [24,260--490].

(a)

(b)

(c)

(d)