Research Article
Immunolocalization of Kisspeptin Associated with Amyloid-β Deposits in the Pons of an Alzheimer’s Disease Patient
Figure 1
Binding of anti-Aβ, anti-KP, anti-catalase and anti-CRH antibodies to Aβ, KP, NPFF, catalase and CRH. In (a) plates were coated with Aβ 1–42, Aβ 17–40, KP 1–54, KP 45–54, NPFF, catalase, and CRH. Binding of BAM-10 mouse anti-Aβ antibody (open columns), rabbit anti-Aβ 21–32 antiserum (closed columns), rabbit anti-KP 45–54 antiserum (open hatched columns), CAT-505 mouse anti-catalase antibody (closed hatched columns) and KCHMB001 mouse anti-CRH antibody (open cross-hatched columns) was detected using alkaline phosphatase conjugated secondary antibodies and p-nitrophenylphosphate substrate. In (b) plates coated with Aβ 1–42 fibrils were preincubated with KP 45–54, NPFF, catalase, or CRH before addition of BAM-10 mouse anti-Aβ antibody (open columns), rabbit anti-Aβ 21–32 antiserum (closed columns), rabbit anti-KP 45–54 antiserum (open hatched columns) and CAT-505 mouse anti-catalase antibody (closed hatched columns), KCHMB001 mouse anti-CRH antibody (open cross-hatched columns). Binding was detected using alkaline phosphatase conjugated secondary antibodies and p-nitrophenylphosphate substrate. Results are expressed as mean ± SEM () absorbance change at 405 nm. (* = versus control; one-way ANOVA).
