Research Article

Dependence of Spreading and Differentiation of Mesenchymal Stem Cells on Micropatterned Surface Area

Figure 5

(a) Phase contrast photographs of MSCs after culture on micropatterned surfaces in osteogenic induction medium for 21 days. (b) Representative optical photographs of ALP staining of MSCs with different spreading areas after culture in osteogenic induction medium for 7 and 21 days. (c) The percentage of osteogenic differentiation of MSCs with different spreading areas after culture in osteogenic induction medium for 7 and 21 days. Data represent mean ± SD ( ). * , ** , and *** . Scale bars: 50 μm.
265251.fig.005a
(a)
265251.fig.005b
(b)
265251.fig.005c
(c)