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Journal of Nanomaterials
Volume 2011 (2011), Article ID 469685, 6 pages
Particle Size Control of Y2O3:Eu3+ Prepared via a Coconut Water-Assisted Sol-Gel Method
Laboratory of Advanced Ceramic Materials, Physics Department, Federal University of Sergipe, 49100-000 São Cristóvão, SE, Brazil
Received 2 October 2010; Revised 24 November 2010; Accepted 23 December 2010
Academic Editor: Yanan Du
Copyright © 2011 Maria de Andrade Gomes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Eu3+-doped Y2O3 nanoparticles were produced through proteic sol-gel technique, and the adjustment of pH was tested in order to control the particle size of the powders. A strong correlation between the initial pH and the temperature of crystallization was observed, allowing the production of particles with controlled diameter from 4 nm to 50 nm. The samples were characterized by X-ray diffraction, high-resolution transmission electron microscopy, optical microscopy and photoluminescence spectroscopy in both absorption and emission modes. A blue shift of the excitation peak corresponding to energy transfer from Y2O3 host to Eu3+ ions was observed, as the particle size was reduced from 50 to 4 nm. The suppression of a charge transfer band also resulted from the reduction of the particle size. The emission spectrum of the Y2O3:Eu3+ with particles of 50 nm was found to be similar to that of bulk material whereas 4 nm particles presented broadened emission peaks with lower intensities.
There is currently a great deal of interest on the development of luminescent nanoparticles for biomedical applications. Compared to traditional organic dyes and fluorescent proteins, inorganic phosphors can offer several advantages such as tunable emission from visible to infrared wavelengths, large absorption coefficient across a wide spectral range, high quantum yield and stability against photobleaching . In the past few years, luminescent quantum dots (QDs) have been investigated aiming their application as labels in biosensing and imaging [2–6]. However, one of the major concerns in the medical applications of QDs is their inherent toxicity, since the most widely used QDs probes, like CdSe, CdTe, CdS, PbSe and ZnSe, are heavy metal-based materials. A number of mechanisms, such as free radical formation of heavy metal, interaction of QDs with intracellular components and surface oxidation, have been postulated to be responsible for QDs cytotoxicity [5, 6]. Considering these limitations, the production of heavy metal-free luminescent nanoparticles becomes a matter of the highest importance and rare earth-doped nanocrystals are pointed out as the best candidates to substitute QDs luminescent probes [7–10].
Amongst a variety of luminescent rare earth-doped systems, Eu3+-doped Y2O3 shows the highest quantum efficiency (almost 100%), and it is widely investigated for applications in fluorescent lamps, display panels and luminescent inks [11–13]. Different methods have been employed to produce nanocrystalline Y2O3:Eu3+ [14–16]. In this work, a coconut water-based sol-gel synthesis, also known as proteic sol-gel route, was investigated. The advantages of this new method are the simplicity and low environmental impact, since it employs the protein chains of coconut water and metal salts in substitution of alcoxide precursors .
As it is widely known, the reactivity of the particles increases as their size decreases. This high reactivity leads to the agglomeration of these small particles and can result in undesirable coalescence  during thermal treatments for the crystallization of the samples. On the other hand, there are reports on the influence of pH on the agglomeration degree and particle size of the materials produced by wet chemistry and combustion synthesis [19–21]. In a previous work, the proteic sol-gel route was used, without pH control, to produce Y2O3 . The smallest particles obtained by this method sized 50 nm. In the present work, the adjustment of the pH during the synthesis was tested in order to produce even smaller particles, establishing a direct relationship between the initial pH and the final granulometry of the powders. The influence of the particle size on the luminescent properties of these phosphors are also presented and discussed.
The Y2O3:Eu3+ nanopowders were prepared via proteic sol-gel  using the salts Y(NO3)3 and Eu(NO3)3 (Alfa Aesar, grade purity 99.99%) as precursor materials. These reactants were mixed with processed coconut water, according to the formula (Y0.98Eu0.02)2O3, and homogenized by stirring at room temperature. In this route, the metal ions are believed to bind to the polymeric chains present in coconut water, forming a colloidal suspension (sol). To adjust pH, ammonium hydroxide, NH4OH (Vetec, P.A.) was added drop by drop into the sol, while it was stirred, and the pH values of the sol were monitored using a Lutron 206 pH meter. It has been reported that the pH of the solution can modify the size of nanoparticles [19–21]. The pH values investigated in this work were 5.0, 7.0, and 9.0. A sample without pH control was also produced and used as reference. After stirring for 10 minutes, the sol was polymerized and formed a gel, which was dried at 100°C/24 hours. The dried material (aerogel) was homogenized and calcined to reach the crystalline phase. Several calcination temperatures were tested from 300°C up to 850°C. After the calcinations, the samples were washed in distilled water to eliminate residual KCl phase, which is an ionic salt present in coconut water.
The crystalline phase of the powders was inspected by X-ray diffraction (XRD-Rigaku RINT 2000/PC) in continuous scanning mode using Cu Kα radiation. The average crystallite size of the samples was obtained from the full width at half maximum (FWHM) of the diffraction peaks, with instrumental correction using LaB6 standard powder [20, 23]. Transmission electron microscopy (TEM) images were obtained with a Tecnai 20 microscope and they were used to analyse morphology and size of the produced nanoparticles. Micrographs of the samples illuminated with ultraviolet (UV) light were obtained with an optical microscope. For both electron and optical microscopy, the powders were dispersed in isopropyl alcohol with the aid of an ultrasonic bath.
The optical properties of the Eu3+-doped Y2O3 samples were inspected via photoluminescence technique in emission and excitation modes. These optical spectra were acquired at room temperature in an ISS PC1TM spectrofluorometer that uses a 300 W Xenon lamp as excitation source. The emission spectra were measured with excitation fixed at 245 nm whereas the excitation spectra were measured by monitoring the intensity of luminescence at 614 nm ( transition of Eu3+).
3. Results and Discussion
Figure 1 presents the X-ray diffraction patterns of 2% Eu3+-doped Y2O3 nanocrystals produced at pH = 7.0 and pH = 9.0. For all the samples studied, the obtained XRD patterns were consistent with the cubic phase of Y2O3 with spatial group Ia3 (JCPDS No 79-1257). The most intense peaks correspond to the crystal planes (222), (400), (440), and (622). These peaks are broadened due to the small size of the nanoparticles. From the diffraction patterns, one can observe that the sample produced at pH = 7.0 was crystallised after calcination at 350°C. At this temperature, all the other samples were amorphous. For the sample synthesized at pH = 9.0, the lowest crystallization temperature was 400°C and the other samples only presented crystalline structure after calcinations at 450°C.
The crystallite size could be estimated from the FWHM of the diffraction peaks using Scherrer’s equation , where is the shape coefficient for the reciprocal lattice point (in this work ), is the wavelength of the X-rays ( nm), is the peak position, and is the width of specimen’s peak () corrected by a instrumental broad factor (in this work, rad). The results are presented in Table 1, where it can be observed that the biggest crystallite size were obtained for the sample calcined at 850°C, departing from the sol without pH control. The smallest crystallites were produced at pH = 7.0 and °C. As expected, the samples calcined at lower temperatures presented smaller crystallites. One can also observe that the particles produced at pH = 7.0 present a pronounced growth at 500°C, reaching the average size of 18 nm whereas the samples produced at lower pH values remain smaller than 10 nm at this temperature.
The reduction of the surface energy is the driving force for both nucleation and particle growth. This thermodynamic parameter depends on the surface tension of curved interfaces and also on the net surface area of the particles . Nanoparticles have high surface energy due to the small curvature radius and large surface area, so the system spontaneously tends to decrease the interface solid solution, and particles tend to grow in order to minimize the surface energy. On the other hand, the surface tension (and, consequently, the surface energy) of particles in suspension can be decreased by adsorption of the amine groups from the ammonium hydroxide , which promote both electrostatic and steric stabilization of nanoparticles. This stabilization prevents the grown of the particles, so the acidity of the medium of synthesis strongly influences the particle size . Besides that, as temperature can also influence surface energy , lowering the synthesis temperature results in a wider size distribution with an increased amount of small particles.
Figure 2 presents TEM images of the powder prepared at pH = 7.0 and treated at 500°C. In Figure 2(a), one can observe spherical-like particles with reduced agglomeration degree when compared with the results from other synthesis methods . For the samples shown in Figure 2, the average particle size observed in the micrographs was 20 nm, which agrees with the crystallite size determined from the X-ray diffraction patterns (see Table 1). Figure 2(b) presents the high-resolution (HRTEM) image of the same sample, where one can observe the lattice planes indicating that each particle contains only one crystallite. Consequently, the crystallite sizes presented in Table 1 can be interpreted as the average particle size of the samples. In Figure 2(b), the distance between family planes is 3.067 Å, which is consistent with the value of 3.066 Å obtained from crystallographic database .
One important step on the development of alternative nontoxic nanocrystals for biomedical imaging is the investigation of their luminescence properties. In this work, the emission and excitation spectra of doped Y2O3:Eu3+ produced by proteic sol-gel were measured for samples with different particle sizes. Figure 3 presents the emission spectra for samples with particle sizes of 50 nm (curve a), 7 nm (curve b), and 4 nm (curve c), under excitation of 245 nm. In these spectra, the intensity was normalized by the excitation intensity (), so the luminescent intensities are comparable. For particle sizes of 50 nm, the features of the emission spectrum are similar to those of the bulk material . The main peak at 614 nm originates from forced electric-dipole transition of Eu3+; the one centered at 633 nm corresponds to transition and the peaks around 595 nm correspond to transition, which is a magnetic-dipole transition . For the samples with average diameter of 7 and 4 nm, the emission peaks are at the same positions, indicating the same electronic transitions, but they are significantly broadened due to local disorder surrounding the Eu3+ ions. The intensity of luminescence also depends on the particle size and this dependence should be taken into account for the application of these nanoparticles as biological labels. At 614 nm, the intensity of the 4 nm particles is 3.3% of that observed for the 50 nm particles. On the other hand, the dimensional similarity of the smaller particles with some biological macromolecules such as nucleic acids and proteins would allow a better integration of these nanoparticles with biological systems, with possible applications in medical diagnostics and targeted therapeutics. Figure 4 presents the images obtained with optical microscope (magnification of 400x) of the powder samples with particle diameter of 50 nm (Figure 4(a)) and 4 nm particles (Figure 4(b)), under UV excitation. Due to the low magnification, it is not possible to distinguish isolated particles, but from these images, it can be concluded that the luminescence of the smaller particles is still strong enough to be detected by fluorescence microscopy techniques.
The excitation spectra for the transition of Eu3+ is presented in Figure 5 for the samples with particle sizes of 50 nm, 18 nm and 4 nm. For smaller particles, it is observed a lower overall intensity of the spectra, compared to the 50 nm sample. An excitation band near 214 nm was observed for all the samples and was assigned to the energy transfer excitation from the Y2O3 host to Eu3+ . This band is slightly blue shifted to 209 nm for the 4 nm sample. For the particles with average size of 50 nm and 18 nm, it is also observed a band at around 210 nm, which is associated with a charge transfer (CT) from O2- to Eu3+. Electrons from the 2p orbital of oxygen are transferred to the excited level of Eu3+, and the red light is emitted when the decay occurs . As the CT band is especially sensitive to the local order surrounding O2-, its intensity is reduced for the particles with 18 nm and suppressed for the particles with 4 nm. Similar behaviour was ascribed by other authors to Y2O3:Eu3+ particles smaller than 10 nm .
Size-controlled Y2O3:Eu3+ nanoparticles were successfully obtained via proteic sol-gel with pH adjustment at the initial stage of the synthesis. The average particle size was tunable from 4 to 50 nm by the control of the pH and calcination temperature of the samples. The advantages of this synthesis route are the simplicity, low environmental impact, and low cost of production, since it employs a natural resource (coconut water) instead of the conventional metallic alkoxides. The temperature of 350°C, used for the production of particles with average diameter of 4 nm, is at least 500°C lower than those reported in the literature for the crystallization of this material . The luminescent characterization of the samples has shown a dependence of the quantum yield on the particle size, probably related to the suppression of the charge transfer from the oxygen to the excited level of Eu3+. Nevertheless, the particles with diameter of 4 nm still presented strong red emission, suggesting that they are suitable to be used in conjugation with biological systems. These conjugation tests are currently being performed in our research group.
The authors wish to acknowledge CETENE for the use of microscopy facilities and the financial agencies CNPq, CAPES, FINEP, and FAPITEC/SE.
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