Efficient In Vitro TRAIL-Gene Delivery in Drug-Resistant A2780/DDP Ovarian Cancer Cell Line via Magnetofection
Figure 1
(a) Gel retardation and (b) Zeta-potential analysis on the complexation of polyMAG-1000/plasmid at varying v/w ratios; (c) Effect of a static magnetic field on gene transfection of pCMV-TRAIL-GFP plasmid towards A2780/DDP cells. The A2780/DDP cells were transfected with polyMAG-1000/plasmid, at the v/w ratio of 1/1, in the absence (w/o) or presence (w/) of a static magnetic field. As a control, the A2780/DDP cells were also transfected with lipofectamine 2000. (d) Transfection efficiency was determined by flow cytometry analysis (*). Results are shown as means and SD values of three independent experiments.
