MRI Contrast Agent-Based Multifunctional Materials: Diagnosis and Therapy

Figure 6

Mesenchymal stem cells (MSCs) labeled with alkyl-PEI/SPIO nanocomposites. Prussian blue staining of unlabeled MSCs (a) and SPIO-labeled MSCs (b) (7 mg Fe/mL, 24 h). The morphology of these SPIO-labelled MSCs is not changed, and blue granules were evident in the cytoplasm around the nuclei; CLSM investigation of the SPIO-labeled MSCs before (c) and after merging with nuclei (d). Electron microscopy of cells labelled with SPIO probes (arrows) (e, f). Magnification (a, b), scale bar 1/4 10 mm (c, d), scale bar 1/4 50 nm (e), scale bar 1/4 100 nm (f) (see [48]).