Nanoparticles of Conjugated Methotrexate-Human Serum Albumin: Preparation and Cytotoxicity Evaluations

Table 1

Preparation and characterization variables and parameters of MTX-HSA conjugate nanoparticles.

Sample	MTX (mg)	HSA (mg)	EDC used for MTX conjugation (mg)	MTX/HSA molar ratio	Particle diameter (nm)	Zeta Potential (mV)	Polydispersity
1	20	250	15	12±0.12	150.3±5.1	−16±0.2	0.20±0.01
2	20	350	15	8±0.18	111.7±4.6	−12±0.5	0.10±0.01
3	20	700	15	2±0.09	95.5±3.2	−9±0.2	0.12±0.01