Research Article

Nanobiosensor for Detection and Quantification of DNA Sequences in Degraded Mixed Meats

Table 1

Probe and target oligo sequences used.

NameSequences (5′→3′)

Porcine-probe (thiolated)TMR-A6-CTG ATA GTA GAT TTG TGA TGA CCG TAG-A6-(CH2)6-SH
Porcine-probe (nonthiolated)TMR-A6-CTG ATA GTA GAT TTG TGA TGA CCG TAG- A6
Porcine-target (complementary)CTA CGG TCA TCA CAA ATC TAC TAT CAG
Porcine-target (noncomplementary)ACG TAA CTG CTG TGG CCA GGT CGC TGA
Porcine-target (single mismatch)CTA CGG TCA TCA CAA ATT TAC TAT CAG

Single mismatch base is shown in underlined bold letter.