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Journal of Nanomaterials
Volume 2011 (2011), Article ID 932719, 8 pages
http://dx.doi.org/10.1155/2011/932719
Research Article

Dynamic Probing of Nanoparticle Stability In Vivo: A Liposomal Model Assessed Using In Situ Microdialysis and Optical Imaging

1Department of Physics, National Chung Hsing University, Taichung 420, Taiwan
2Division of Medical Engineering Research, National Health Research Institutes, Zhunan, Miaoli 350, Taiwan
3Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu 300, Taiwan
4Department of Chemistry, National Tsing Hua University, Hsinchu 300, Taiwan
5Center for Nanomedicine Research, National Health Research Institutes, Zhunan, Miaoli 350, Taiwan

Received 7 June 2010; Revised 10 August 2010; Accepted 6 September 2010

Academic Editor: Libo Wu

Copyright © 2011 Chien-Chung Jeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nanoparticle-mediated drug delivery and controlled release has been a vigorous research area in contemporary nanomedicine. The in vivo stability of nanoparticle delivered on site is a prerequisite for the design of drug-controlled release by any means. In this study, the first methodology comprised of microdialysis and optical imaging to assess the liposome stability in vivo is reported. Macroscopically, we demonstrated the DPPG liposomes with negative surface charge fast accumulated in the rat liver upon their i.v. administration using optical imaging. Microscopically, the concurrent analysis of fluorescent molecules leaching from the liposomes, in situ sampled using microdialysis probe, provides the dynamic information of stability of DPPG liposomes locus in quo. The current combination of in situ microdialysis and optical imaging possesses a great potential for use as a platform technology to evaluate the nanoparticle stability and the bioavailability of drug payload released on targeted site in vivo.