Characterization of Plasmid DNA Location within Chitosan/PLGA/pDNA Nanoparticle Complexes Designed for Gene Delivery
Figure 4
Gel analysis of complex formation and degradation. (a) Complexes formed at various ratios: lane 1: 100 ng plasmid DNA; lane 2: 1 : 1 plasmid:particle complex; lane 3: 0.1; lane 4: 0.05; lane 5: 0.02; lane 6: 0.01; lane 7: 1 kb ladder. (b) Plasmid and pDNA/NP complexes after nuclease digestion using DNase I: lane 1: 1 kb ladder; lane 2: 100 ng plasmid; lane 3: 0.02 complex; lane 4: 100 ng plasmid with DNase I; lane 5: 0.02 complex with DNase I. (c) Plasmid and pDNA/NP complexes treated with chitosanase and lysozyme: lane 1:1 kb ladder; lane 2: 100 ng plasmid; lane 3: 0.02 plasmid:particle complex; lane 4 : 100 ng plasmid with chitosanase/lysozyme; lane 5: 0.02 complex with chitosanase/lysozyme.
