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Journal of Nanomaterials
Volume 2012 (2012), Article ID 265161, 7 pages
http://dx.doi.org/10.1155/2012/265161
Research Article

Characterization and Antiproliferative Activity of Nobiletin-Loaded Chitosan Nanoparticles

1Centro de Investigación en Alimentación y Desarrollo AC, Coordinación Regional Guaymas, Carret. al Varadero Nacional Km 6.6, 85480 Guaymas, SON, Mexico
2Centro de Investigación en Alimentación y Desarrollo AC, Coordinación Hermosillo, Carret. a la Victoria Km 0.6, 83304 Hermosillo, SON, Mexico
3Institut für Biologie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universtät-Münster, 48149 Münster, Germany
4Departamento de Física, Universidad de Sonora, 83000 Hermosillo, SON, Mexico
5Laboratorio de Investigación en Bioactivos y Alimentos Funcionales, Rubio Pharma y Asociados S.A. de C.V., 83210 Hermosillo, SON, Mexico
6Centro de Investigación Científica de Yucatán, 97200 Mérida, YUC, Mexico

Received 6 June 2012; Revised 20 August 2012; Accepted 21 August 2012

Academic Editor: Fathallah Karimzadeh

Copyright © 2012 Ana G. Luque-Alcaraz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nobiletin is a polymethoxyflavonoid with a remarkable antiproliferative effect. In order to overcome its low aqueous solubility and chemical instability, the use of nanoparticles as carriers has been proposed. This study explores the possibility of binding nobiletin to chitosan nanoparticles, as well as to evaluate their antiproliferative activity. The association and loading efficiencies are 69.1% and 7.0%, respectively. The formation of an imine bond between chitosan amine groups and the carbonyl group of nobiletin, via Schiff-base, is proposed. Nobiletin-loaded chitosan nanoparticles exhibit considerable inhibition (IC50=8μg/mL) of cancerous cells, revealing their great potential for applications in cancer chemotherapy.