Basic Potential of Carbon Nanotubes in Tissue Engineering Applications

Figure 4

MWCNT treatment induces cellular lipid accumulation in C2C12 cells. Cells were treated with 2% horse serum (HS) (A) and 10% FBS (C) or 10 μg/mL MWCNT (E and G) for 7 days after the cells reached 70–80% confluence. Oil red O staining was performed after 7 days of exposure to the growth medium. The arrows indicate cells positive for lipid droplet content. Cell nuclei (indicated as black arrows) stained with Hoechst 33342 (blue) were visualized on day 7 by using a fluorescent microscope (B, D, F, and H). (I) Lipid accumulation was quantitatively assayed with a lipid-staining reagent to evaluate the lipid content [47].