- About this Journal ·
- Abstracting and Indexing ·
- Aims and Scope ·
- Annual Issues ·
- Article Processing Charges ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Recently Accepted Articles ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Journal of Nanomaterials
Volume 2012 (2012), Article ID 398720, 7 pages
Enhanced Oxidative Stress and Physiological Damage in Daphnia magna by Copper in the Presence of Nano-TiO2
Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, Beihang University, Beijing 100191, China
Received 9 May 2012; Accepted 23 May 2012
Academic Editor: Jiaguo Yu
Copyright © 2012 W. H. Fan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study examines the potential hazard of an individual nanomaterial on the Cu biotoxicity to aquatic organisms. Daphnia magna in the absence or presence of nano-TiO2 was exposed to Cu. Maintaining nano-TiO2 at a safe concentration cannot eliminate its potential hazard. The biomarkers superoxide dismutase, catalase, and Na+/K+-ATPase in D. magna were measured. Cu in the presence of nano-TiO2 induced higher levels of oxidative stress and physiological damage because of the sorption of Cu. Nano-TiO2 also caused Na+/K+-ATPase inhibition possibly by impeding the Na+/K+ transfer channel. The correlations among the biomarkers, mortality, and accumulation further showed that the overloading reactive oxygen species generation caused by nano-TiO2 contributed to deeper oxidative stress and physiological regulation, thereby causing greater toxic injury.
Nanotechnology has attracted considerable attention in the scientific community since its emergence as a powerful basic and applied science tool [1, 2]. The production of engineered nanomaterials was estimated to reach 2000 tons in 2004 and is expected to increase to 58 000 tons in 2011–2020 . With increasing commercialization of nanoparticles (NPs), concerns about the exposure of humans and the environment to NPs are growing . The unique sizes of NPs result in many special physicochemical properties and may yield extraordinary hazards for human health and the environment .
The biological toxicity of NPs is closely related to many physicochemical characteristics such as size, surface area, surface modification, and radical formation. The consideration of these properties in assessing NP toxicity complements the conventional dose- (concentration-) response approach. Nano-TiO2 is globally important as a sunscreen and pigment, and its physicochemical properties are widely documented [2, 5, 6]. Size is an important factor that determines nano-TiO2 toxicity because penetration becomes easier with decreasing particle size. Bioavailability toward the sites to be taken up is also increased; thus, more particles can be deposited inside the cell . Nano-TiO2 particles smaller than 25 nm cause higher algal growth inhibition and greater immobilization of Daphnia magna than those bigger than 100 nm . Exposure to 0.22 m filtered nano-TiO2 also causes higher mortality in D. magna than exposure to unfiltered nano-TiO2, indicating that toxicity may be directly related to the size of the dispersed NPs . Some ecological studies showed that nano-TiO2 exposure in aquatic species causes oxidative damage-mediated effects [10, 11]. The exposure of rainbow trout to nano-TiO2 causes lipid peroxidation, one of the consequences of oxidative stress ; changes in antioxidant enzyme activities are also observed in freshwater cladoceran (Daphnia pulex) . However, biochemical studies on the effect of nano-TiO2 on oxidative stress, which has been proposed as an important biochemical biomarker, remain limited to aquatic vertebrate species, and the effect of nano-TiO2 size fraction has not been investigated. A study on D. magna indicated the importance of the colloidal behavior and mode of preparation of nano-TiO2 to resultant toxicity , and the lethal concentration of nano-TiO2 is only 10 ppm for D. magna following 48 h of aqueous exposure . However, little is known about the biological effects under exposure to safer concentrations.
The increasing use of engineered NPs in industrial and household applications may very likely lead to the release of such materials into the environment . At least one study reported enhanced mobility of engineered NPs in simulated groundwater systems . The unique structure and electronic properties of nano-TiO2 can make it an especially powerful adsorbent . Little is currently known about the fate, transport, and transformation of NPs once they enter the environment . Several studies employing colloidal behavior have investigated the transport of a wide range of engineered NPs through porous media . Colloidal behavior can help predict the behavior of NPs released into the aquatic environment . Zhang et al.  reported that nano-TiO2 can change the uptake of other pollutants and found that carp exposed to cadmium in the presence of nano-TiO2 accumulated 146% more Cd than controls.
A previous study revealed that the coexistence of NPs with copper ion (Cu2+) enhances the biotoxicity of Cu2+ to daphnids even at low concentrations . In the current study, we measured a range of end points, including biochemical measurements related to physiological functions (e.g., Na+/K+-ATPase) and oxidative stress (e.g., superoxide dismutase (SOD) and catalase (CAT)). Na+/K+-ATPase is a member of the P-type ATPase family of cation transporters , which belongs to a superfamily of ubiquitous pumps involved in the transport of charged substrates across biological membranes . Decreased Na+/K+-ATPase activity in cells may impede ion transfer across membranes and cause disorder in the metabolism of substances and energy. SOD activity is sensitive to tissue copper (Cu) because the enzyme requires Cu as a catalytic cofactor. Cu deficiency can also decrease the activities of certain non-Cu-containing enzymes of the oxidant defense system, including CAT . The biotoxicity of Cu2+ is reportedly correlated with the interactions between its adsorption and coordination with cosubstrates . Consequently, we used Cu to interfere with nano-TiO2 and assessed the ecological impact of nano-TiO2 on aquatic organisms. Therefore, the aforementioned biomarkers were used to reveal the potential risk of the combination of nano-TiO2 with Cu2+.
2.1. Preparation of Nano-TiO2 Suspension
Nano-TiO2 particles (anatase) were provided by Nanjing High Technology Material Co., Ltd. The N2-BET-specific surface area was measured using a Nova 2200e BET surface area analyzer (Quantachrome, Boynton Beach, FL) at 114.45 m2 g−1. Under the aforementioned conditions, the particle size was about 13.5 nm. The stock suspension of nano-TiO2 (20 mg L−1) was prepared based on the procedure described by Lovern and Klaper , in which 2 mg of nano-TiO2 particles was mixed with 100 mL of deionized H2O and then placed in a bath sonicator for at least 30 min to break the particles into small, noncoagulating particles. The stock solution was stored at room temperature before usage. The image of nano-TiO2 particles in water and the adsorption of Cu2+ onto the nano-TiO2 were previously studied . Using a capillary tube, droplets of solution were injected onto the Formvar-coated (Electron Microscopy Sciences, Fort Washington, PA, USA) copper grids. The solution was allowed to dry, and the samples were then placed in the TEM for imaging. The image of nano-TiO2 particles in water was observed by a JEOL transmission electron microscope (TEM) (JEOL, JEM-2100F), operated at 100 kV electron volts. The image result has been shown in Figure 1.
2.2. Model Organism
Daphnia magna used in this study was cultured at 23°C with a light : dark cycle of 16 : 8 h 2 years after collecting the algae from natural waters near Huo Qi Ying Bridge (116°16′732 E, 39°58′401 N). The green alga Scenedesmus obliquus was fed to the daphnids at a concentration of 1 × 105–2 × 105 cells mL−1 d−1. The algae were grown in artificial WC medium  and collected by centrifugation at the exponential growth stage. The water used for all exposure experiments was also collected from the Huo Qi Ying Bridge area. The physicochemical parameters of the water were as follows: pH 7.6, Ca2+ concentration of 24 mg L−1, total organic carbon concentration of 5.23 mg L−1, and dissolved oxygen concentration of 11.3 mg L−1. The water used in all instances was filtered through a 0.45 m membrane before use.
2.3. Exposure of D. magna to Cu in the Absence or Presence of Nano-TiO2
Daphnia magna (21–25 d) was exposed to different concentrations of dissolved Cu2+ (as copper nitrate) in the absence or presence of nano-TiO2 particles for 3 d. The concentrations of Cu2+ used in the study were 10, 20, 30, 40, 50, 70, and 100 g L−1. Thirty D. magna of the same age in 200 mL to 300 mL of water were used in each exposure treatment, with two replicates for each treatment. Daphnia magna was not fed during its exposure period. The numbers of dead individuals were noted each day, and the mortality rate was calculated at the end of the exposure. A control test without Cu2+ contaminant was also conducted under the same conditions.
2.4. Determination of SOD, CAT, and Na+/K+-ATPase in D. magna
Twenty exposed daphnids were weighed after removing the water on their body surfaces. Tissues of D. magna were homogenized by ultrasonication in 0.5 mL of sucrose buffer (0.25 M sucrose and 0.1 M Tris-HCl, pH 8.6) and centrifuged at 16 000 × g for 20 min. The supernatant fluid was diluted to 1.5 mL using a homogenate, and 1 mL of supernatant fluid was used to determine SOD, CAT, and Na+/K+-ATPase using commercially available kits (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer’s protocol. SOD activity was based on the inhibition by SOD of NADPH oxidation by molecular oxygen in the presence of EDTA, manganese chloride, and 2-mercaptoethanol . CAT activity was calculated and expressed as nmol H2O2 consumed s−1 g−1 protein−1 . Na+/K+-ATPase was assessed based on the amount of inorganic phosphate liberated from the hydrolysis of the substrate ATP .
3. Results and Discussion
3.1. Induction of Oxidative Stress by Cu in the Absence or Presence of Nano-TiO2
The main potential mechanism of NP toxicity is believed to be via oxidative stress with reactive oxygen species (ROS), which damages lipids, carbohydrates, proteins, and DNA. To interpret the differences between the toxicity of Cu only and Cu2+ adsorbed onto nano-TiO2 (Cu + nano-TiO2), SOD and CAT activities in D. magna were detected during the exposure (Figure 2). Cell toxicity is principally induced by oxidative stress ; thus, the SOD and CAT activities in D. magna were investigated because they are antioxidant biomarkers for metal pollution. The SOD and CAT activities decreased to different extents. These activities were significantly () induced in the groups with and without nano-TiO2 compared with the control. Figure 2(a) shows that in the presence of nano-TiO2, the highest induction (208.2% of the control) was reached at 10 g L−1, whereas in the absence of nano-TiO2, the highest induction (203.3% of the control) was at 20 g L−1. The induction then decreased proportionally with increased Cu2+ concentration. The SOD activities had no significant difference between the two groups (, one-way ANOVA). The activity of CAT, a part of the SOD-CAT system that defends against oxygen toxicity , differed from that of SOD. Figure 2(b) showed that the CAT activities in the Cu2+-exposed D. magna were significantly higher than those in the Cu2+/nano-TiO2-exposed D. magna (, one-way ANOVA). In the presence of nano-TiO2, the highest induction (368.9% of the control) was reached at 10 g L−1, whereas in the absence of nano-TiO2, the highest induction (504.81% of the control) was at 20 g L−1. The induction then decreased proportionally with increased Cu2+ concentration. The presence of nano-TiO2 reduced the CAT activity in D. magna, and the largest observed drop was 55.7%. The antioxidant enzyme activities increased at the nano-TiO2 concentration of 5 mg L−1 . However, at Cu concentrations greater than 40 g L−1 and at a safe nano-TiO2 concentration of 2 mg L−1, the activity of the entire antioxidant-system (SOD + CAT) considerably decreased, indicating decreased antioxidant capacity in D. magna and suggesting that Cu + nano-TiO2 was more dangerous than Cu alone in aquatic environments.
3.2. Inhibition of Na+/K+-ATPase by Cu in the Absence or Presence of Nano-TiO2
Figure 3 shows that compared with the group without nano-TiO2, the group with nano-TiO2 exhibited a statistically significant decrease in Na+/K+-ATPase activities, with a reduction range between 21.3% and 45.3% (, one-way ANOVA). Na+/K+-ATPase indicates the ability of ion transfer in the cell membrane channel. Na+/K+-ATPase enzyme is present at high concentrations in salt-transporting tissues such as intestines and gills, where it maintains the ionic and electrical gradients necessary for transepithelial salt movements . Santore et al.  had proposed that Cu2+ accumulation in the gills of freshwater fish inhibits Na+ influx and reduces Na+/K+-ATPase activity. Exposure to Cu2+ leads to concentration-related losses of plasma ions , particularly sodium and chloride, and damaged gill structure and function . Given that Na+/K+-ATPase activity tends to compensate for effects at the gill by showing normal activity when branchial Na+/K+-ATPase activity is low, Na+/K+-ATPase inhibition did not result in plasma Na+ or K+ depletion. Therefore, Cu + nano-TiO2 has an inhibitive effect on the antioxidant enzyme (Figure 3).
3.3. Mechanism of Enhanced Biotoxicity of Cu2+ by Nano-TiO2
In invertebrate species, SOD and CAT are considered to play greater antioxidant roles . Figure 4(a) shows that the SOD activity and accumulated Cu had a definite positive correlation (, one-way ANOVA). The relationship between SOD activity and mortality in D. magna also had a significant correlation (, one-way ANOVA) (Figure 4(b)). In the groups with and without nano-TiO2, the CAT activity decreased significantly (, one-way ANOVA) with increased accumulated Cu (Figure 4(c)). The mortality also decreased significantly (, one-way ANOVA) with increased CAT activity (Figure 4(d)). Normally, Cu participates in the formation of ROS. In the presence of superoxide (), Cu2+ can be reduced to Cu+, which is capable of catalyzing the formation of hydroxyl radical (OH•) from hydrogen peroxide (H2O2) . The hydroxyl radical is the most powerful oxidizing radical and is capable of reacting with practically every biological molecule and destroying the antioxidant defense system . The relationship between the oxidative stresses at the organismal (e.g., uptake) and biochemical levels in daphnids indicated that in the SOD-CAT system, free Cu ions and Cu + nano-TiO2 may produce the same level of because the induced SOD and CAT activities had a definite positive correlation with accumulated copper. Therefore, Cu + nano-TiO2 induced Cu biotoxicity by oxidative stress (Table 2). Cu can generate ROS (e.g., , H2O2, and OH•) only in digestive tissues . Thus, Cu + nano-TiO2 must enter digestive tissues to generate ROS according to Figure 4. Cu + nano-TiO2 generated the devastating ROS, which destroyed the antioxidant defense system. Ultimately, the overload of ROS damaged the daphnid as the induced SOD-CAT system capability dropped to a very low level.
To discuss further the mechanism of the enhanced biotoxicity of Cu + nano-TiO2, the relationship between Cu accumulation and Na+/K+-ATPase was analyzed. As shown in Figure 5(a), the presence of nano-TiO2 led to decreased Na+/K+-ATPase level. Cu2+ and Cu2++nano-TiO2 exposure was differentiated by the inhibition of Na+/K+-ATPase in D. magna. Therefore, we speculated that the function of this enzyme was inhibited due to the damage caused by Cu  and nano-TiO2 . The reason was that nano-TiO2 is a powerful adsorbent and may compete with the active binding sites for essential elements of organisms such as K+ and Ca2+. Therefore, the Na+/K+-ATPase activity was affected. Cu2+ can cause the inhibition of Na+/K+-ATPase enzyme activity by interfering with the binding of Cu2+ and protein-thiol. The binding site of Cu2+ has special interactions. However, Federici et al.  found that Na+, K+, and Ca2+ in fish tissues were generally unaffected, and exposure to nano-TiO2 caused a statistically significant decrease in intestinal Na+/K+-ATPase activity. Therefore, we speculated that nano-TiO2 may have impeded the Na+/K+ transfer channel due to its small particle size , which increased the biotoxicity of physiological effects compared with the system without nano-TiO2, including the inhibition of ion transfer across the membrane and disturbance of the metabolism of substances and energy. Consequently, the inhibition worsened.
Considering both exposure systems together, the relationship between mortality and Na+/K+-ATPase in D. magna had no significant correlation (Figure 5(b)). By contrast, considering the two systems independently, the mortality and Na+/K+-ATPase activity in each system had a positive correlation, that is, the mortality of D. magna decreased significantly with increased Na+/K+-ATPase level. The observed levels of Na+/K+-ATPase were explained by the toxicity of Cu ions in D. magna. The function of Na+/K+-ATPase is considered to respond to physiological function in aquatic organisms; thus, the observed decline in Na+/K+-ATPase indicated physiological effect inhibition. At the same mortality level, Na+/K+-ATPase inhibition by Cu + nano-TiO2 was lower than that by Cu only. Given that ROS generation was uncontrollable because of the breakdown of antioxidant action, we believed that the protective response was inactivated and overtaken by inflammation and cytotoxicity. Therefore, these defects or aberrations can determine disease susceptibility during the exposure, and worsened Na+/K+-ATPase inhibition by Cu + nano-TiO2. Finally, the high ROS concentration fatally induced damage to cell structures, lipids, and proteins, and the aquatic organisms died from digesting the mixture of toxicants.
Even at low and safe levels, nano-TiO2 can enhance oxidative stress by ROS generation due to its high adsorbability. Cu + nano-TiO2 generated ROS and the antioxidant defense system was damaged due to the inhibition by Cu or Cu + nano-TiO2. Nano-TiO2 was able to impede the Na+/K+ transfer channel because of its particle size, thus causing Na+/K+-ATPase inhibition. The increased ROS generation caused by Cu + nano-TiO2 led to higher toxicity. These ROS led to higher inhibition of Na+/K+-ATPase and physiological functions were damaged. These results indicated that the sorption of NPs played an important role in their toxicity to aquatic organisms. Our study provided one of the first detailed overviews on oxidative stress and the physiological effects of Cu + nano-TiO2 in D. magna and further elucidated nanosafety by revealing the correlation among the antioxidation system, Na+/K+-ATPase, mortality, and bioaccumulation.
This work was supported by China’s National Basic Research Program “Water environmental quality evolution and water quality criteria in lakes” (no. 2008CB418201), Natural Science Foundation of China (no. 40871215), Natural Science Foundation of Beijing (no. 8092019) and the Fundamental Research Funds for the Central Universities.
- A. S. Karakoti, L. L. Hench, and S. Seal, “The potential toxicity of nanomaterials—the role of surfaces,” JOM Journal of the Minerals, Metals and Materials Society, vol. 58, no. 7, pp. 77–82, 2006.
- M. C. Roco and W. S. Bainbridge, “Societal implications of nanoscience and nanotechnology: maximizing human benefit,” Journal of Nanoparticle Research, vol. 7, no. 1, pp. 1–13, 2005.
- A. D. Maynard, “Nanotechnology: the next big thing, or much ado about nothing?” Annals of Occupational Hygiene, vol. 51, no. 1, pp. 1–12, 2007.
- S. J. Klaine, P. J. J. Alvarez, G. E. Batley et al., “Nanomaterials in the environment: behavior, fate, bioavailability, and effects,” Environmental Toxicology and Chemistry, vol. 27, no. 9, pp. 1825–1851, 2008.
- B. Nowack and T. D. Bucheli, “Occurrence, behavior and effects of nanoparticles in the environment,” Environmental Pollution, vol. 150, no. 1, pp. 5–22, 2007.
- M. R. Wiesner, G. V. Lowry, P. Alvarez, D. Dionysiou, and P. Biswas, “Assessing the risks of manufactured nanomaterials,” Environmental Science and Technology, vol. 40, no. 14, pp. 4336–4345, 2006.
- L. K. Limbach, Y. Li, R. N. Grass et al., “Oxide nanoparticle uptake in human lung fibroblasts: effects of particle size, agglomeration, and diffusion at low concentrations,” Environmental Science and Technology, vol. 39, no. 23, pp. 9370–9376, 2005.
- K. Hund-Rinke and M. Simon, “Ecotoxic effect of photocatalytic active nanoparticles (TiO2) on algae and daphnids,” Environmental Science and Pollution Research, vol. 13, no. 4, pp. 225–232, 2006.
- S. B. Lovern and R. Klaper, “Daphnia magna mortality when exposed to titanium dioxide and fullerene (C60) nanoparticles,” Environmental Toxicology and Chemistry, vol. 25, no. 4, pp. 1132–1137, 2006.
- G. Federici, B. J. Shaw, and R. D. Handy, “Toxicity of titanium dioxide nanoparticles to rainbow trout (Oncorhynchus mykiss): gill injury, oxidative stress, and other physiological effects,” Aquatic Toxicology, vol. 84, no. 4, pp. 415–430, 2007.
- R. Klaper, J. Crago, J. Barr, D. Arndt, K. Setyowati, and J. Chen, “Toxicity biomarker expression in daphnids exposed to manufactured nanoparticles: changes in toxicity with functionalization,” Environmental Pollution, vol. 157, no. 4, pp. 1152–1156, 2009.
- Y. Pan, S. Neuss, A. Leifert et al., “Size-dependent cytotoxicity of gold nanoparticles,” Small, vol. 3, no. 11, pp. 1941–1949, 2007.
- S. B. Lovern, J. R. Strickler, and R. Klaper, “Behavioral and physiological changes in Daphnia magna when exposed to nanoparticle suspensions (titanium dioxide, nano-C60, and C60C70),” Environmental Science and Technology, vol. 41, no. 12, pp. 4465–4470, 2007.
- H. F. Lecoanet, J. Y. Bottero, and M. R. Wiesner, “Laboratory assessment of the mobility of nanomaterials in porous media,” Environmental Science and Technology, vol. 38, no. 19, pp. 5164–5169, 2004.
- X. Chen and S. S. Mao, “Titanium dioxide nanomaterials: synthesis, properties, modifications and applications,” Chemical Reviews, vol. 107, no. 7, pp. 2891–2959, 2007.
- H. Sun, X. Zhang, Z. Zhang, Y. Chen, and J. C. Crittenden, “Influence of titanium dioxide nanoparticles on speciation and bioavailability of arsenite,” Environmental Pollution, vol. 157, no. 4, pp. 1165–1170, 2009.
- H. F. Lecoanet and M. R. Wiesner, “Velocity effects on fullerene and oxide nanoparticle deposition in porous media,” Environmental Science and Technology, vol. 38, no. 16, pp. 4377–4382, 2004.
- T. Galloway, C. Lewis, I. Dolciotti, B. D. Johnston, J. Moger, and F. Regoli, “Sublethal toxicity of nano-titanium dioxide and carbon nanotubes in a sediment dwelling marine polychaete,” Environmental Pollution, vol. 158, no. 5, pp. 1748–1755, 2010.
- X. Zhang, H. Sun, Z. Zhang, Q. Niu, Y. Chen, and J. C. Crittenden, “Enhanced bioaccumulation of cadmium in carp in the presence of titanium dioxide nanoparticles,” Chemosphere, vol. 67, no. 1, pp. 160–166, 2007.
- W. Fan, M. Cui, H. Liu et al., “Nano-TiO2 enhances the toxicity of copper in natural water to Daphnia magna,” Environmental Pollution, vol. 159, no. 3, pp. 729–734, 2011.
- G. Crambert, U. Hasler, A. T. Beggah et al., “Transport and pharmacological properties of nine different human Na,K- ATPase isozymes,” The Journal of Biological Chemistry, vol. 275, no. 3, pp. 1976–1986, 2000.
- J. Y. Uriu-Adams and C. L. Keen, “Copper, oxidative stress, and human health,” Molecular Aspects of Medicine, vol. 26, no. 4-5, pp. 268–298, 2005.
- J. Chen, D. Zhu, and C. Sun, “Effect of heavy metals on the sorption of hydrophobic organic compounds to wood charcoal,” Environmental Science and Technology, vol. 41, no. 7, pp. 2536–2541, 2007.
- R. R. L. Guillard, Culture of Marine Invertebrate Animals, Plenum Press, New York, NY USA, 1975.
- F. Paoletti and A. Mocali, “Determination of superoxide dismutase activity by purely chemical system based on NAD(P)H oxidation,” Methods in Enzymology, vol. 186, pp. 209–220, 1990.
- K. Gawehn and H. U. Bergmeyer, Methods of Enzymatic Analysis, Academic Press, New York, NY, USA, 1974, edited by Hans Ulrich Bergmeyer in Collaboration with Karlfried Gawehn.
- P. A. Lanzetta, L. J. Alvarez, P. S. Reinach, and O. A. Candia, “An improved assay for nanomole amounts of inorganic phosphate,” Analytical Biochemistry, vol. 100, no. 1, pp. 95–97, 1979.
- S. Kim, J. E. Choi, J. Choi et al., “Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells,” Toxicology in Vitro, vol. 23, no. 6, pp. 1076–1084, 2009.
- S. Pandey, S. Parvez, I. Sayeed, R. Haque, B. Bin-Hafeez, and S. Raisuddin, “Biomarkers of oxidative stress: a comparative study of river Yamuna fish Wallago attu (Bl. & Schn.),” The Science of the Total Environment, vol. 309, no. 1–3, pp. 105–115, 2003.
- J. P. Morth, B. P. Pedersen, M. J. Buch-Pedersen et al., “A structural overview of the plasma membrane Na+,K+-ATPase and H+-ATPase ion pumps,” Nature Reviews Molecular Cell Biology, vol. 12, no. 1, pp. 60–70, 2011.
- R. C. Santore, D. M. Di Toro, P. R. Paquin, H. E. Allen, and J. S. Meyer, “Biotic ligand model of the acute toxicity of metals. 2. Application to acute copper toxicity in freshwater fish and Daphnia,” Environmental Toxicology and Chemistry, vol. 20, no. 10, pp. 2397–2402, 2001.
- R. D. Handy, F. B. Eddy, and H. Baines, “Sodium-dependent copper uptake across epithelia: a review of rationale with experimental evidence from gill and intestine,” Biochimica et Biophysica Acta, vol. 1566, no. 1-2, pp. 104–115, 2002.
- J. Li, E. S. Quabius, S. E. Wendelaar Bonga, G. Flik, and R. A. C. Lock, “Effects of water-borne copper on branchial chloride cells and Na+/K+-ATPase activities in Mozambique tilapia (Oreochromis mossambicus),” Aquatic Toxicology, vol. 43, no. 1, pp. 1–11, 1998.
- C. Barata, I. Varo, J. C. Navarro, S. Arun, and C. Porte, “Antioxidant enzyme activities and lipid peroxidation in the freshwater cladoceran Daphnia magna exposed to redox cycling compounds,” Comparative Biochemistry and Physiology C, vol. 140, no. 2, pp. 175–186, 2005.
- G. R. Buettner, “The pecking order of free radicals and antioxidants: lipid peroxidation, α-tocopherol, and ascorbate,” Archives of Biochemistry and Biophysics, vol. 300, no. 2, pp. 535–543, 1993.
- T. D. Rae, P. J. Schmidt, R. A. Pufahl, V. C. Culotta, and T. V. O'Halloran, “Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase,” Science, vol. 284, no. 5415, pp. 805–808, 1999.
- A. E. Nel, L. Mädler, D. Velegol et al., “Understanding biophysicochemical interactions at the nano-bio interface,” Nature Materials, vol. 8, no. 7, pp. 543–557, 2009.