Single-Molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Based on Evanescent Field-Enhanced Fluorescence Imaging
Figure 4
Evanescent field-enhanced fluorescence images via prism-type TIRFM of (a) standard AFP (AFP-positive sample), (b) normal (nonpathologic) human serum, and (c) AFP-spiked human serum on gold nanopatterned chip.
