Research Article

Control of In Vivo Transport and Toxicity of Nanoparticles by Tea Melanin

Figure 6

Time course examination by CARS microscopy of hippocampi isolated from mice in the melanin-treated only group (M), the 17 nm GNP-treated group (17 nm), the 37 nm GNP-treated group (37 nm), the melanin pretreatment-plus 17 nm GNP-treated group (M + 17 nm), and the melanin pretreatment-plus 37 nm GNP-treated group (M + 37 nm). The wavelengths of the Pump and Stokes lasers (Pump = 870 nm and Stokes = 1,064 nm) were tuned to match a Raman shift ( 2,100 cm−1) that fell in the so-called “silent region” of the vibrational spectra of cells and tissues. To better visualize the location of GNPs, the enhanced bright spots are colored red in the final images. The green fluorescence is the autofluorescence emitted from the cells of the CA region of the hippocampus.
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