Nanobioprobe for the Determination of Pork Adulteration in Burger Formulations

Figure 1

Schematic presentation of the operating and quantification principles of the swine nanobiosensor probes. Three oligonucleotides probes flanked by a hexyl-A spacer and linked to a gold nanocrystal through a gold-thiol bond at one end and fluorophore (TMR) to the other are shown to be self-organized in a constrained arch-like structure where the fluorophore is quenched by the nanoparticle in the absence of any complementary targets. Upon target binding, the closed structure is opened into a rod-like conformation separating the fluorescent dye from the nanoparticle to allow fluorescence emission. The degree of fluorescence emission is shown to proportionate to the degree of target hybridization. Gold nanoparticle is demonstrated by a red sphere. The quenched and emission state of the fluorophore is represented by a sky-blue sphere and a sky-blue star. Single-stranded probe DNA and target DNA are shown by green- and blue-curved line, respectively.