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Journal of Nanomaterials
Volume 2013 (2013), Article ID 274805, 7 pages
http://dx.doi.org/10.1155/2013/274805
Research Article

Antibody-Conjugated Rubpy Dye-Doped Silica Nanoparticles as Signal Amplification for Microscopic Detection of Vibrio cholerae O1

1Program in Bioscience, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand
2National Institute of Health, Department of Medical Sciences, Tiwanond Road, Nonthaburi 11000, Thailand
3Department of Physics, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand
4Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
5Food Safety Center, King Mongkut’s University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand

Received 12 July 2013; Accepted 29 August 2013

Academic Editor: Do Kim

Copyright © 2013 Nualrahong Thepwiwatjit et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study demonstrated the potential application of antibody-conjugated Rubpy dye-doped silica nanoparticles for immunofluorescence microscopic detection of Vibrio cholerae O1. The particle synthesis of 20X of the original ratio was accomplished yielding spherical nanoparticles with an average size of  nm. The nanoparticles were carboxyl functionalized and then conjugated with either monoclonal antibody or polyclonal antibody against V. cholerae O1. The antibody-conjugated nanoparticles were tested with two target bacteria and three challenge strains. The result showed that monoclonal antibody-conjugated Rubpy dye-doped silica nanoparticles could be effectively used as signal amplification to detect V. cholerae O1 under a fluorescence microscope. Their extremely strong fluorescence signal also enables the detection of a single cell bacterium.