About this Journal Submit a Manuscript Table of Contents
Journal of Nanomaterials
Volume 2013 (2013), Article ID 384717, 11 pages
http://dx.doi.org/10.1155/2013/384717
Research Article

Bioreducible PEI-siRNA Nanocomplex for Liver Cancer Therapy: Transfection, Biodistribution, and Tumor Growth Inhibition In Vivo

1Department of Imaging, Tongji Hospital, Tongji University, Shanghai 200065, China
2Department of Nuclear Medicine, Shanghai Seventh People’s Hospital, Shanghai 200137, China
3Translational Medicine Research Centre and Central Laboratory, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200040, China
4The Institute for Advanced Materials and Nano Biomedicine, School of Medicine, Tongji University, Shanghai 200092, China
5School of Life Sciences and Technology, Tongji University, Shanghai 200092, China

Received 21 May 2012; Revised 11 December 2012; Accepted 11 December 2012

Academic Editor: Takuya Tsuzuki

Copyright © 2013 Wei Xia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. W. Y. Lau, “Primary liver tumors,” Seminars in Surgical Oncology, vol. 19, no. 2, pp. 135–144, 2000.
  2. K. Okuda, “Hepatocellular carcinoma,” Journal of Hepatology, vol. 32, no. 1, pp. 225–237, 2000. View at Scopus
  3. I. O. L. Ng, R. T. P. Poon, J. M. F. Lee, S. T. Fan, M. Ng, and W. K. Tso, “Microvessel density, vascular endothelial growth factor and its receptors Flt-1 and Flk-1/KDR in hepatocellular carcinoma,” American Journal of Clinical Pathology, vol. 116, no. 6, pp. 838–845, 2001. View at Publisher · View at Google Scholar · View at Scopus
  4. R. Yamaguchi, H. Yano, A. Iemura, S. Ogasawara, M. Haramaki, and M. Kojiro, “Expression of vascular endothelial growth factor in human hepatocellular carcinoma,” Hepatology, vol. 28, no. 1, pp. 68–77, 1998. View at Publisher · View at Google Scholar · View at Scopus
  5. H. P. Gerber, V. Dixit, and N. Ferrara, “Vascular endothelial growth factor induces expression of the antiapoptotic proteins Bcl-2 and A1 in vascular endothelial cells,” Journal of Biological Chemistry, vol. 273, no. 21, pp. 13313–13316, 1998. View at Publisher · View at Google Scholar · View at Scopus
  6. T. Takahashi, H. Ueno, and M. Shibuya, “VEGF activates protein kinase C-dependent, but Ras-independent Raf-MEK-MAP kinase pathway for DNA synthesis in primary endothelial cells,” Oncogene, vol. 18, no. 13, pp. 2221–2230, 1999. View at Scopus
  7. M. Shibuya, “Structure and function of VEGF/VEGF-receptor system involved in angiogenesis,” Cell Structure and Function, vol. 26, no. 1, pp. 25–35, 2001. View at Publisher · View at Google Scholar · View at Scopus
  8. G. R. Devi, “siRNA-based approaches in cancer therapy,” Cancer Gene Therapy, vol. 13, no. 9, pp. 819–829, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. J. H. Jeong, L. V. Christensen, J. W. Yockman et al., “Reducible poly(amido ethylenimine) directed to enhance RNA interference,” Biomaterials, vol. 28, no. 10, pp. 1912–1917, 2007. View at Publisher · View at Google Scholar · View at Scopus
  10. E. Raskopf, A. Vogt, T. Sauerbruch, and V. Schmitz, “siRNA targeting VEGF inhibits hepatocellular carcinoma growth and tumor angiogenesis in vivo,” Journal of Hepatology, vol. 49, no. 6, pp. 977–984, 2008. View at Publisher · View at Google Scholar · View at Scopus
  11. Y. K. Oh and T. G. Park, “siRNA delivery systems for cancer treatment,” Advanced Drug Delivery Reviews, vol. 61, no. 10, pp. 850–862, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. D. J. Gary, N. Puri, and Y. Y. Won, “Polymer-based siRNA delivery: perspectives on the fundamental and phenomenological distinctions from polymer-based DNA delivery,” Journal of Controlled Release, vol. 121, no. 1-2, pp. 64–73, 2007. View at Publisher · View at Google Scholar · View at Scopus
  13. C. Boyer, P. Priyanto, T. P. Davis et al., “Anti-fouling magnetic nanoparticles for siRNA delivery,” Journal of Materials Chemistry, vol. 20, no. 2, pp. 255–265, 2010. View at Publisher · View at Google Scholar · View at Scopus
  14. O. Boussif, F. Lezoualch, M. A. Zanta et al., “A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 16, pp. 7297–7301, 1995. View at Publisher · View at Google Scholar · View at Scopus
  15. W. Xia, P. Wang, C. Lin et al., “Bioreducible polyethylenimine-delivered siRNA targeting human telomerase reverse transcriptase inhibits HepG2 cell growth in vitro and in vivo,” Journal of Controlled Release, vol. 157, no. 3, pp. 427–436, 2012.
  16. W. Xia and C. Lin, “Bioreducible polymer-delivered siRNA targeting human telomerase reverse transcriptase for human cancer gene therapy,” Therapeutic Delivery, vol. 3, no. 4, pp. 439–442, 2012.
  17. C. Lin and J. F. J. Engbersen, “PEGylated bioreducible poly(amido amine)s for non-viral gene delivery,” Materials Science and Engineering C, vol. 31, no. 7, pp. 1330–1337, 2011.
  18. Y. Y. Sun, Z. F. Deng, Y. Tian, and C. Lin, “Horseradish peroxidase-mediated in situ forming hydrogels from degradable tyramine-based poly(amido amine)s,” Journal of Applied Polymer Science, vol. 127, no. 1, pp. 40–48, 2013.
  19. C. Lin and J. P. Ge, “Multifunctional polyethylenimine-conjugated superparamagnetic nanoparticles for drug delivery and imaging,” Journal of Controlled Release, vol. 152, supplement 1, pp. e58–e60, 2011.
  20. F. Li, Q. J. Ba, S. M. Niu et al., “In-situ forming biodegradable glycol chitosan-based hydrogels: synthesis, characterization, and chondrocyte culture,” Materials Science & Engineering C, vol. 32, no. 7, pp. 2017–2025, 2012.
  21. C. Lin and J. F. J. Engbersen, “The role of the disulfide group in disulfide-based polymeric gene carriers,” Expert Opinion on Drug Delivery, vol. 6, no. 4, pp. 421–439, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. C. Lin, Z. Zhong, M. C. Lok et al., “Novel bioreducible poly(amido amine)s for highly efficient gene delivery,” Bioconjugate Chemistry, vol. 18, no. 1, pp. 138–145, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. C. Lin, C.-J. Blaauboer, M. M. Timoneda et al., “Bioreducible poly(amido amine)s with oligoamine side chains: synthesis, characterization, and structural effects on gene delivery,” Journal of Controlled Release, vol. 126, no. 2, pp. 166–174, 2008. View at Publisher · View at Google Scholar · View at Scopus
  24. C. Lin, Z. Y. Zhong, M. C. Lok et al., “Linear poly(amido amine)s with secondary and tertiary amino groups and variable amounts of disulfide linkages: synthesis and in vitro gene transfer properties,” Journal of Controlled Release, vol. 116, no. 2, pp. 130–137, 2006. View at Publisher · View at Google Scholar · View at Scopus
  25. C. Plank, K. Mechtler, F. C. Szoka, and E. Wagner, “Activation of the complement system by synthetic DNA complexes: a potential barrier for intravenous gene delivery,” Human Gene Therapy, vol. 7, no. 12, pp. 1437–1446, 1996. View at Scopus
  26. S. Son, K. Singha, and W. J. Kim, “Bioreducible BPEI-SS-PEG-cNGR polymer as a tumor targeted nonviral gene carrier,” Biomaterials, vol. 31, no. 24, pp. 6344–6354, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. S. Son and W. J. Kim, “Biodegradable nanoparticles modified by branched polyethylenimine for plasmid DNA delivery,” Biomaterials, vol. 31, no. 1, pp. 133–143, 2010. View at Publisher · View at Google Scholar · View at Scopus