Table 1: Expanded design summary of goldfish (Carassius auratus).

ParameterControlGroup AGroup B

Volume of water in aquarium (L)1010 10
TiO2 NPs concentrations (mg/L)010100
Duration of exposure (day)555
Water temperature (°C)
Oxygen (ppm)
pH value of water (Start–End)6.30–6.056.03–6.156.63–6.45
Number of fish within the aquarium555
Number of replications222