Nanoporous Aluminium Oxide Membranes as Cell Interfaces
Table 2
Growth of microorganisms on prepatterned and surface-modified AAO nanopores.
Surface modification
Type of micropattern or coating on AAO nanopores
Microorganism type
Coating
Reference
Micropattern with physical barriers
(i) Commercial Anodisc AAO chips (Whatman) with 200 nm pores were coated with Ordyl 314 acrylic film (Elga Europe) (ii) Structuring via RIE created microwells from μm2 to μm2.
Lactobacillus plantarum WCFS1, Escherichia coli XL2 Blue, and Candida albicans JBZ32
(i) Customised AAO nanopores produced via two-step anodisation (50 nm) (ii) Structuring PEG coating via photolithography yielded circular microcompartments with diameters of 80 um
(i) Commercial Anodisc chips with 200 nm pore diameter (Whatman) (ii) Contact printing of microorganisms with PDMS stamps (iii) Cells were printed on untreated and on AAO membranes compartmentalized into μm2 culture areas by acrylic plastic walls covered with a 20 nm layer of platinum
Lactobacillus plantarum, Escherichia coli, Aspergillus fumigatus, and several strains of Candida