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Journal of Nanomaterials
Volume 2013 (2013), Article ID 473703, 6 pages
http://dx.doi.org/10.1155/2013/473703
Research Article

DNA Detection of Toxoplasma gondii with a Magnetic Molecular Beacon Probe via CdTe@Ni Quantum Dots as Energy Donor

1State Key Laboratory of Hollow Fiber Membrane Materials and Membrane Process, Tianjin Polytechnic University, Tianjin 300382, China
2School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300382, China

Received 24 March 2013; Accepted 14 June 2013

Academic Editor: Sebastian Mackowski

Copyright © 2013 Shichao Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A new method for detection of Toxoplasma gondii via DNA sensing technology was developed in this study. It was based on the mechanism of fluorescence resonance energy transfer (FRET) in which multifunctional and magnetic-fluorescent CdTe@Ni quantum dots (mQDs) were utilized as energy donor and a commercial BHQ2 as acceptor. The sensing probe was fabricated by labeling a stem-loop Toxoplasma gondii DNA oligonucleotide with CdTe@Ni mQDs at the 5′ end and BHQ2 at 3′ end, respectively. The surface assembly of CdTe on Ni core and the formation of CdTe@Ni were confirmed by XRD analysis. The sizes of CdTe, Ni nanoparticles, and CdTe@Ni were measured via TEM and XRD methods and estimated to be ~3 nm, ~15 nm, and ~20 nm, respectively. The sensing ability was investigated by the fluorescence spectrum (FS). An obvious fluorescence recovery was observed when the complete complimentary target Toxoplasma gondii DNA was introduced, which did not happen in the case of the target DNA with one-base pair mismatch. Our research indicates that the current sensing probe is sensitive and specific in detection of Toxoplasma gondii DNA and has great potential in Toxoplasmosis diagnosis.