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Journal of Nanomaterials
Volume 2013 (2013), Article ID 751236, 4 pages
http://dx.doi.org/10.1155/2013/751236
Research Article

Detection of Carbofuran with Immobilized Acetylcholinesterase Based on Carbon Nanotubes-Chitosan Modified Electrode

1College of Science, University of Shanghai for Science and Technology, Shanghai 200093, China
2College of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093, China

Received 4 January 2013; Accepted 14 February 2013

Academic Editor: Yongfeng Luo

Copyright © 2013 Shuping Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A sensitive and stable enzyme biosensor based on efficient immobilization of acetylcholinesterase (AChE) to MWNTs-modified glassy carbon electrode (GCE) with chitosan (CS) by layer-by-layer (LBL) technique for rapid determination of carbofuran has been devised. According to the inhibitory effect of carbamate pesticide on the enzymatic activity of AChE, we use carbofuran as a model pesticide. The inhibitory effect of carbofuran on the biosensor was proportional to concentration of carbofuran in the range from  g/L to  g/L with a detection limit of  g/L. This biosensor is a promising new method for pesticide analysis.