Effects of Multiwalled Carbon Nanotube Reinforced Collagen Scaffolds on the Osteogenic Differentiation of Mesenchymal Stem Cells

Table 1

List of qRT-PCR primers.

Gene	Description	Primer	𝑇𝑚
GAPDH	Glyceraldehyde 3-phosphate dehydrogenase	Forward: 5′ TCGGTGTGAACGGATTTGG 3′; Reverse: 5′ TCTCCACTTTGCCACTGCA 3′	55.1°C; 57.4°C
BGLAP	Osteocalcin	Forward: 5′ CTGACAAAGCCTTCATGTCCAA 3′; Reverse: 5′ GCCGGAGTCTGTTCACTACCTT 3′	55.2°C; 58.3°C
RunX	Transcription factor RunX2	Forward: 5′ CTTCAAGGTTGTAGCCCTCGGA 3′; Reverse: 5′ ATGACGGTAACCACAGTCCCA 3′	58.4°C; 58.5°C
AP	Alkaline phosphatase	Forward: 5′ AAGGATATCGACGTGATCATGG 3′; Reverse: 5′ GGCCTTCTCATCCAGTTCGTA 3′	54.1°C; 56.7°C
Smad4	SMAD family member 4	Forward: 5′ CACAAGTCAGCCGGCCAGTATT 3′; Reverse: 5′ TTCCAGTCCAGGTGGTAGT 3′	59.4°C; 59.0°C