Abstract

In this work we have synthesized and characterized new hybrid nanoplatforms for luminescent biolabeling based on the concept of Eu3+ complexes encapsulation in mesoporous silica nanoparticles (100 nm). Eu complexes have been selected on the basis of their capability to be excited at 365 nm which is a currently available wavelength, on routine epifluorescence microscope. For Eu complexes encapsulation, two different routes have been used: the first route consists in grafting the transition metal complex into the silica wall surface. The second way deals with impregnation of the mesoporous silica NPs with the Eu complex. Using the second route, a silica shell coating is realized, to prevent any dye release, and the best result has been obtained using Eu-BHHCT complex. However, the best solution appears to be the grafting of Eu(TTA)3-Phen-Si to mesoporous silica NPs. For this hybrid, mSiO2-Eu(TTA)3(Phen-Si) full characterization of the nanoplatforms is also presented.

1. Introduction

Recent breakthroughs in the synthesis of mesoporous silica materials with the control of the particle size, the morphology, and the porosity, along with their chemical stability, have made silica matrices highly attractive as the structural basis for a wide variety of nanotechnological applications such as adsorption, catalysis, sensing, and separation [17]. In addition, some authors have highlighted that surface-functionalized mesoporous silica nanoparticle (MSN) materials can be readily internalized by animal and plant cells without posing any cytotoxicity issue in vitro [8, 9]. These new developments offer the possibility of designing a new generation of drug/gene delivery systems and biosensors for intracellular controlled release applications.

Another possible application consists in encapsulating a luminescent dye in plain or mesoporous silica nanoparticles for optical biolabeling [1012]. For this goal, the dye molecule must be perfectly trapped inside the mesoporous matrix in order to prevent the leaching and bleaching effects. Many dye molecules can be encapsulated inside mesoporous NPs; however, we think that lanthanide complexes as Eu3+ or Tb3+ have the strong competitive advantage (versus commercial organic probes) to allow the time delayed measurement for complete extinction of the biological self-fluorescence during the measurement [13]. Moreover, encapsulated lanthanide chelates are not, or weakly, subjected to photobleaching or photobleaking, they are chemically very stable and non-toxic. All these factors constitute major advances, as it has been well demonstrated by Dr. Jin and his team [14].

However, for most luminescent Eu3+ and Tb3+ complexes, one of the major drawbacks is that optical excitation window is limited to the far-UV (<330 nm) range. Far-UV excitation is often problematic in biology because it causes damages to the cellular matter. Excitation below 330 nm involves poor transmission in most optics, is bulky, expensive, and has limited light sources. Most of commercial flow cytometers and microscopes are not used at these wavelengths [1416].

Several longer-wavelength-sensitized Eu3+ complexes have been developed in recent years [1722] and used as biolabels for time-resolved luminescence bioimaging applications. Their properties have been illustrated, for example, by the highly specific and sensitive imaging of an environmental pathogen, that is, Giardia lamblia [23], and by the use of bioconjugated silica nanoparticles embedding an europium complex to mark cancerous cells [24]. However for our knowledge, encapsulation of such complexes inside mesoporous nanoparticles has not been done systematically. The main advantage of mesoporous NPs is their high loading capability compare to plain SiO2 NPs, which can potentially lead to brighter probes. Consequently, the main goal of this work is to encapsulate these new long wavelength-sensitized Eu3+ complexes and to characterize the derived new nanohybrids, for cellular labeling, using light excitation in the NUV range (355–365 nm) given by laser (or Hg lamp) sources available on flow cytometer or fluorescent microscope. To reach this goal we have used two different approaches. The first route consists in grafting transition metal complexes into the silica wall surface by using a bifunctional ligand which can chelate the metal on one side and react with the silica wall on the other side (samples named as mSiO2-XXX in the following). The second way is easier and consists in impregnating the mesoporous silica NPs with the Eu3+ complex and then to elaborate a silica shell coating which will prevent any dye release (samples named as mSiO2@XXX in the following).

2. Experimental Section

2.1. Reagents and Materials

Most reagents were purchased from Sigma-Aldrich. N,N′-Dimethylformamide (DMF), hexane, chloroform, and ethanol were of analytical grade and used without any further purification. Eu(NO3)3 was aqueous stock solution from Rhodia.

2.2. Chemical Synthesis
2.2.1. Synthesis of Mesoporous Silica Nanoparticles (mSiO2)

Typically, 0.2821 g of NaOH (PRS Panreac) and 1.048 g of cetyltrimethylammonium bromide (CTAB) were mixed with 480 mL of distilled water. After this, the mixture was kept under constant stirring, and the temperature was increased up to 80°C. 5 mL of tetraethyl orthosilicate (TEOS) was added as the silica precursor, dropwise, slowly (in 20 min approximately). The mixture was kept at 80°C under vigorous stirring for 20 h. The obtained precipitate was centrifuged and washed with water. The sedimented product was rapidly dried in an oven at 60°C and then treated at 500°C for 5 h (increase 1°C/min), in order to decompose all the surfactants. The final weight of the obtained silica was approximately 1.0 g.

2.2.2. Synthesis of Eu(TTA)3(Phen-Si) and Grafting in mSiO2

Eu(TTA)3(Phen-Si) complex was prepared via a two-step process as shown in Figure 1.

(a) Synthesis of Ligand Phen-Si. The ligand was prepared according to the procedures described by Li et al. [25, 26]. Typically, 5-amino-1,10-phenanthroline (4.1 mmol, 800.4 mg) was dissolved in CH3Cl (75 mL), and 3-(triethoxysilyl)propyl isocyanate (4.5 mmol, 1.11 mL) was added to the solution. The mixture was then reduced to a volume of 5 mL and refluxed at 65°C under Ar overnight. Cold hexane was then added to precipitate the powder. This powder was collected by centrifugation, washed with hexane, and dried under vacuum overnight.

Elemental analysis for C22H30N4O4Si, %, found (calcd.): C 52.8 (57.4); H 5.7 (6.22); N 12.1 (13.4). 1H NMR (300.13 MHz; CDCl3, ppm) 0.63 (2H, m, CH2, 14-H), 1.10 (9H, t, 7, CH3, 16-H), 1.68 (2H, m, CH2, 13-H), 3.34 (2H, m, CH2, 12-H), 3.75 (6H, q, 7, CH2, 15-H), 6.34 (1H, br, CH, 7-H), 7.20 (1H, dd, CH, 3′-H), 7.57 (1H, dd, CH, 3-H), 8.15 (1H, m, CH, 4′-H), 8.18 (1H, br, NH, 11-H), 8.37 (1H, m, CH, 4-H), 8.38 (1H, br, NH, 9-H), 8.86 (1H, m, CH, 2′-H), 9.00 (1H, m, CH, 2-H). 13C{1H} NMR (75.5 MHz; CDCl3, ppm) 7.7 (s, CH2, 14), 18.3 (s, CH3, 16), 23.7 (s, CH2, 13), 42.8 (s, CH2, 12), 58.4 (s, CH2, 15), 118.1 (s, CH, 7), 122.4 (s, CH, 3′), 123.5 (s, CH, 3), 125.0 (s, C, 6), 129.0 (s, C, 5′), 131.0 (s, CH, 4′), 132.8 (s, C, 5), 135.9 (s, CH, 4). 143.0 (s, C, 6′), 146.2 (s, C, 8), 149.0 (s, CH, 2′), 149.6 (s, CH, 2), 156.8 (s, C, 10).

(b) Synthesis of Complex Eu(TTA)3(Phen-Si). The complex was prepared according to the procedure described by Duan et al. [28]. 2-Thenoyltrifluoroacetone (TTA) (6 mmol, 1.332 g) was dissolved in 20 mL of absolute ethanol, and triethylamine (6 mmol, 0.81 mL) was added. After 10 min of stirring, the ligand (Phen-Si) (2 mmol, 0.738 g) was added, followed by Eu(NO3)3 (2 mmol, 0.517 g). The reaction mixture was heated at 50–60°C and stirred under Ar for an appropriate time (3–5 h). The reaction mixture was then cooled to room temperature, and the solvent was removed at 40°C under reduced pressure until obtaining a powder. Then the powder was washed with water and centrifuged. The crude product was then recrystallized by refluxing in ethanol to obtain the desired product which was collected by filtration and dried under vacuum at 40°C.

Elemental analysis for C46H39N4O10F9S3SiEu, %, found (calcd.): C 41.4 (44.0); H 3.6 (3.1); N 8.2 (8.16). SM. (m/z); found 839.9. Calc. for (Eu(Phen-Si)(TTA)(NO3))+: 837. IR (KBr, cm−1): 2945  (CH2, CH3); 2808  (CH2, CH3); 1546, 1500 ν (C=O); 1440, 1423, 1385  (C–C=C) aromatic; 737, 668 γ (=C–H) aromatic.

(c) Grafting of Eu(TTA)3(Phen-Si) into mSiO2 Nanoparticles. The grafting was carried out according to a modified protocol from Rocha et al. [27]. mSiO2 NPs were suspended in DMF. 94.2 μmol/g of Eu(TTA)3(Phen-Si) complex was also suspended with DMF. Then the two suspensions were mixed, and the final concentration of mSiO2 was 1 mg/mL. The mixture was refluxed for 24 h. The powder was then centrifuged, washed three times with ethanol, and dried at 80°C in an oven overnight. The europium content, determined by TEM-EDX, is 0.1% (mol).

2.2.3. Synthesis of Si-DBM-Eu(DBM)2 Complex and Grafting in mSiO2

(a) Preparation of Sodium β-Diketonate (Na-DBM). The ligand was prepared according to the procedure described by Machado et al. [29] and De Oliveira et al. [30]. Na(s) (0.7 g, 30.0 mmol) was dissolved in 30 mL of anhydrous methanol under an argon atmosphere to produce sodium methoxide. 6.7 g (30.0 mmol) of dibenzoylmethane (DBM) was added to the methoxide solvent to obtain a viscous suspension. Subsequently, the powder was collected by filtration, washed with anhydrous methanol, and dried under vacuum at 50°C producing Na-DBM with a yield of 85%.

(b) Synthesis of Silylant Agent with 3-Chloropropyltrimethoxysilane (TMOSCl). TMOSCl (1.13 mL, 6.0 mmol) and 1.482 g (6.0 mmol) of Na-DBM were added to 30 mL of anhydrous methanol. The solution was stirred under argon atmosphere at 50°C for 24 h. The silylating agent was denoted by Na(Si-DBM). Figure 2 shows the chemical structure that represents this process.

(c) Grafting of Na(Si-DBM) inside mSiO2 Nanoparticles, Complexation with Eu3+. The grafting was carried out according to a modified protocol from Rocha et al. [27]. mSiO2 NPs (50 mg) were added to anhydrous ethanol (0.35 mol) and 30% NH4OH (10 mmol) mixture. Na(Si-DBM) solution (1.31 mL) was then added to the above mixture that was then stirred for 1 h. The powder was then centrifuged, washed three times with ethanol, and dried at 50°C in an oven overnight. Finally, the powder was suspended in anhydrous ethanol (10 mL) containing EuCl3 (2.35 mL, 0.10 mol·L−1), producing mSiO2-Eu(Si-DBM). To complete the coordination sphere of Eu3+, 20 mg of DBM-Na was added to produce the final luminescent material, SiDBM-Eu(DBM)2 [30]. The powder was again centrifuged, washed three times with ethanol, and dried at 50°C in an oven overnight.

FTIR spectrum of sample is presented Figure 3. The typical bands of both DBM and silica structure, such as a large band centered on 3434 cm−1, assigned to OH stretching of silanol groups of inorganic mesoporous structure of material and also adsorbed and/or bonded water can be seen. Three peaks at 2964, 2923, and 2852 cm−1 are related to C–H stretching of CH2 and CH3 groups. The mesoporous silica structure can also be seen with the bands at 1065, 805, and 455 cm−1, corresponding to the different Si–O–Si vibrations (stretching, bending, and rocking, resp.). Signal related to the beta-diketone can also be observed with the four bands at 1597, 1548, 1458, and 1313 cm−1, corresponding, respectively, to (C=O), ν (C=C), (C=O), and (C–C) of DBM [30]. Europium content has been quantified by EDX-MET and has been found to be 0.04% (mol).

2.2.4. Impregnation of mSiO2 NPs with Eu(DBM)3(Phen) and Eu(BHHCT) Complexes

(a) Synthesis of Eu(DBM)3(Phen) and mSiO2 Impregnation. The complex was prepared according to Melby et al. [31] with some modifications. The ligand was first deprotonated by addition of 113.6 μL of a solution of KOH in methanol 0.990 mol·L−1 to 750 μL of an ethanolic solution of dibenzoylmethane (DBM) 0.150 mol·L−1, followed by 375 μL of an ethanolic solution of 1,10-phenantroline 0.1 mol·L−1. Then, 375 μL of an aqueous solution of Eu(NO3)3 0.1 mol·L−1 was added dropwise under magnetic stirring, to give the precipitated complex [Eu(DBM)3(Phen)]. This suspension was stirred for 24 h at room temperature and then centrifuged. The precipitate was carefully washed with ethanol, recovered by centrifugation, and dried at 60°C in air overnight. The complex was then dissolved in DMSO and stirred with 30 mg of mesoporous silica nanoparticles during 24 h at room temperature, in order to encapsulate it. The amount of europium complex impregnated was calculated to be 6.25 μmol per 5 mg of mesoporous silica. Finally, the sample was coated with silica-amine shell as described in the next topics. After analysis, the Eu3+ content has found to be 0.42% (mol).

(b) Synthesis of Eu(BHHCT) Complex and mSiO2 Impregnation. The ligand 4,40-bis(1′′,1′′,1′′,2′′,2′′,3′′,3′′-heptafluoro-4′′,6′′-hexanedion-6′′-yl) chlorosulfo-o-terphenyl (BHHCT) was prepared as previously reported [32]. In order to form the complex Eu-BHHCT, 5 mg (6.25 μmol) of BHHCT ligand was dissolved in 15 mL of propanol. 2.29 mg (6.25 μmol) of EuCl3·6H2O was dissolved in 0.25 mL of distilled water and then added to the BHHCT solution. The mixture was aged at room temperature in darkness, in order to form the complex. Then, 5 mg of mesoporous silica nanoparticles was added to the complex solution, and the suspension was kept under stirring for one night, at room temperature, in darkness. In order to prevent any leak of the impregnated complex, the silica coating was elaborated without any purification. However, after silica coating, Eu3+ content has been determined by MET-EDX and was found to be 1% (mol).

(c) Aminosilane Coating. In order to avoid the leak of the impregnated complexes, the nanocapsules were closed by coating them with a thin silica layer. Typically 30 mg of impregnated silica nanoparticles was dissolved in 80 mL of propanol under ultrasound for 2 h. Then, 8.94 mL of NH4OH (28%), 7.5 mL of distilled water, and 25 μL of TEOS were added to the mixture and stirred at 40°C for 2 h. Then, 100 μL of (3-aminopropyl)trimethoxysilane (APTMS) was added and the mixture stirred for 1 more hour. The reaction mixture is then centrifuged and washed with propanol, and the obtained precipitate is dried in oven overnight. The final weight of the material was approximately 45 mg. Note that the APTMS molecule does not play any role to prevent the dye release. This molecule has been added at the end of the coating protocol in order to introduce amine functions which are very useful for further biofunctionalization.

2.3. Physical and Chemical Characterizations

1H and 13C NMR spectra were recorded on Bruker Advance 300, with chemical shifts (in ppm) reported relative to tetramethylsilane. Mass spectra were recorded by FAB or IS techniques using a Normas R10-10 spectrometer. Elemental analyses were performed on elementary analyses (EA) which were performed using a Perkin Elmer 2400 series II elemental analyser. Chemical bonding was characterized by infrared spectroscopy using a Perkin Elmer spectrometer 100 series. Samples were prepared by mixing the powders with potassium bromide (1/100 by weight) in a pellet. Nitrogen adsorption-desorption curves were measured with a Belsorp-mini (BEL Japan Inc.) between 0 and 99 p/p0 at 77 K. Pretreatment was performed under vacuum during 24 h at 80°C. Small angle X-ray scattering (SAXS) analyses were performed on an INEL XRG3D device. Small angle X-ray scattering signal from mesoporous silica was obtained with X-rays produced by a Cu anode. The X-ray beam was then filtered and focused onto the specimen using Kirkpatrick-Baez mirrors, thus delimiting a small and nondivergent beam. Scattered intensity was recorded on an imaging plate, located 38 cm behind the specimen. Particle shape, size, and composition were examinated by Transmission Electron Microscopy (TEM) using Philips CM20 FEG microscope, equipped with EDX detector. This EDX detector was used to quantify Eu contents of samples. Fluorescence spectra were recorded with a Fluorolog FL3-22 Jobin Yvon spectrometer equipped with a R928 Hamamatsu photomultiplier and a 450 W excitation lamp. For the analysis of emission decay versus time, a pulsed Xe source was employed. The emission decays have been recorded under excitation at 365 nm, monitoring the 5D07F2 at its maximum (612 nm). Experimental decays have been calculated according the formula , with an error range estimated to be 15%.

2.4. Cell Culture, Cytotoxicity Test, and Fluorescence Imaging

An indirect cytotoxicity test was performed using an elution method as described previously [33]. The used cells are MDA-MB231 which are triple negative breast cancer cells [34]. The cells are maintained in culture in RPMI 1640 medium complemented with 10% fetal bovine serum, 1% penicillin-streptomycin and incubated at 37°C with 5% CO2. For in vitro labeling as for cytotoxic tests, cells were placed in 96-well plate at 10000 cells/well. The particles were added at different concentrations to the cell medium after sonication. The MTT (methyl thiazoletetrazolium, Sigma) test is used to evaluate the viability of the MDA-MB231 in the presence of different concentrations of the NPs (mSiO2-Eu(TTA)3(Phen-Si)) one and three days after their addition to cell culture medium. MTT test is a colorimetric assay for measuring the activity of enzymes that reduce MTT to formazan dye, giving a purple color. DMSO (dimethylsulfoxide) solution is added to dissolve the insoluble purple formazan product into a colored solution. The absorbance of this colored solution can be quantified by measuring at 570 nm by a spectrophotometer.

For in vitro labeling, cells were incubated with NPs (0.1 mg/mL, 24 h). Microscopic images were obtained using a “home-made” Time Gated Luminescence Microscope (TGLM) kindly built for us by Dr. Dayong Jin from Macquarie University of Sydney. The main interest of a TGLM is to be able to separate long-lasting fluorescence coming from lanthanides from self-fluorescence coming from the biological media [23].

3. Results and Discussion

3.1. Synthesis of Mesoporous Silica Nanoparticles

The synthesis procedure is based on the protocol proposed by [35]. After a full optimization procedure with many varying parameters as reactant concentration (TEOS: 0.93 mmol to 4.7 mmol and CTAB: 0.77 mmol to 7.1 mmol), temperature (25 to 90°C), and reaction time (2 to 20 h), we find that the best results (average size close to 100 nm, spherical shape, no agglomeration, high surface area, and a high porous volume) were obtained with the procedure reported in Section 2. Figure 4 shows SEM images (a) of particles. Average particle size (feret diameter, counted on 242 particles) is 116 nm with a standard deviation of 45 nm. On the TEM image of Figure 4(b) one can clearly see the well-ordered mesoporous structure of particles. SAXS analysis presented in Figure 5 is characteristic of a hexagonal MCM 41 well-organized mesostructure [36] with three visible diffraction peaks: d(100) = 3.37 Å, d(110) = 2.22 Å, and d(200) = 1.91 Å. The adsorption/desorption isotherm (BET) experiments done at 77 K under nitrogen give a specific surface area equal to 1018 m2 g−1. The average pore size is centered at 5.476 nm whereas total porous volume is estimated at 1.397 cm3 g−1.

3.2. Comparison of Luminescent Properties of Eu Complexes Incorporated in mSiO2

In order to verify that no release of complexes occurs in aqueous solution we have checked that nanoplatforms do not lose luminescence intensity (<5%) after severe water leaching (3× 1 h in water). Then, to compare performance of the different luminescent nanoplatforms we have recorded an emission spectrum after excitation at 365 nm (laser diode or Hg lamp wave length usually found on many epifluorescence microscope) under exactly the same condition (0.25 mg·mL−1 of NPs in water). Results, presented in Figure 6, show that all complexes have a maximum emission band centred around 613 nm. However, emission intensities, recorded under the same conditions, are different. The sample presenting the most intense luminescence is the one with Eu(TTA)3(Phen-Si) complex, grafted inside the mesopores of the mSiO2 NPs. As this sample presents the highest luminescent intensity, it has been selected for further characterization.

3.3. Characterization of the Eu(TTA)3(Phen-Si) Complex Grafted in mSiO2

Eu(TTA)3(Phen-Si) complex was successfully prepared via a two steps process as shown in Figure 1. Ligand (Phen-Si) was first obtained by reacting 5-amino-1,10-phenanthroline and 3-(triethoxysilyl)propyl isocyanate (Figure 1(a)). Europium complex was then prepared from Eu(NO3)3, 2-thenoyltrifluoroacetone (TTA), and phenantroline ligand (Phen-Si) in the presence of triethylamine in ethanol at 50–60°C (Figure 1(b)). Figure 7 shows infrared spectra of Eu(TTA)3(Phen-Si) complex, mSiO2, and the sample of mSiO2 incorporating the complex (mSiO2-Eu(TTA)3(Phen-Si)). The spectrum of the complex presents the characteristic bands of phenanthroline as well as those of TTA, indicating that the complex has been obtained. On the mSiO2 spectrum, the well-known bands of SiO2 are observed. The mSiO2-Eu(TTA)3(Phen-Si) spectrum exhibits both bands of the complex and mSiO2, especially in the region of 1700–600 cm−1.

The sample mSiO2-Eu(TTA)3(Phen-Si) has also been investigated by BET analysis. After loading, the porous volume decreases down to 0.9570 m3·g−1 (instead of 1.397 m3·g−1), as well as the specific area to 684 m²·g−1 (instead of 1018 m²·g−1). This loss of porous volume and specific area confirms that the complex Eu(TTA)3(Phen-Si) is well grafted into the mesopores of the NPs.

Figure 8 presents the elemental cartography obtained by EDX spectroscopy on STEM microscopy. It can be seen that after incorporation, mSiO2 keeps its spherical shape without any aggregation. EDX spectroscopy results show that silicon atoms are homogenously dispersed to form the mesoporous silica matrix (Figure 8(b)). Nitrogen and europium atoms are also detected, corresponding to the grafted complex (Figures 8(c) and 8(d)). Images reveal that these elements are homogenously well dispersed all inside the NPs, confirming the good repartition of the complex, all around the walls of the mesoporous silica.

In Figure 9(a) emission spectra of the grafted complex recorded in ethanol after excitation at 365 nm are gathered. For the free complex the concentration was 0.25 mg·mL−1, corresponding to 2·10−4 mol·L−1 in Eu3+, and for the grafted complex the concentration was 1 mg·mL−1, corresponding to 2·10−5 mol/L in Eu3+ (considering a grafting rate of 0.1% (mol)). The characteristic emission lines of transitions 5D07 of Eu3+ are observed for both samples. Some differences may be noticed: for instance, the shape of the 5D07F1 transition and relative intensities of 5D07F4/5D07F2. Figure 9(b) presents excitation spectra for these samples observed at 612 nm. For the free complex, one broad band centred on 360 nm can be seen. After incorporation into mSiO2 this broad band remains, but seems to be shifted to higher energy, around 330 nm. This is probably due to the covalent grafting of the complex into mSiO2, which modifies energy transfers from the antenna to Eu3+ ions. The 5D0 luminescence decays for the free complex and the complex grafted into mSiO2 are shown in Figure 9(c). The free complex exhibits monoexponential decay with lifetime  ms. For grafted complex, the decay is clearly biexponential: the faster component is the same as for pure complex, but a slower component with lifetime estimated to about 1 ms is also observed. The average decay, estimated with the formula , is  ms. From the comparison of emission spectra and of emission decays, at least two populations of Eu3+ are then observed after grafting into mSiO2. A detailed investigation of luminescence data, necessary to discuss the possible structures of these populations, is beyond the scope of this paper. It is important to notice here two essential features for the potential applications. The first point is the red emission observed for the dispersed NPs and the pure complex in solution, both excited at 365 nm, that is, in the organic antenna, and recorded under the same experimental conditions and have the same intensities. The other point of interest is that the emission lifetime of the grafted NPs is suitable for microsecond time gated detection of luminescence.

3.4. Cytotoxicity Tests on Nanoparticles

The optical density (OD) is directly proportional to the living cells number. The comparison of the proliferation of MDA-MB231 cancer cells, in contact (during 3 days) with growing concentrations of NPs 0.1; 0.5; 1; and 2 mg/mL, emphasizes a significant decrease of cell viability, and an inhibition of cell growth for doses of NPs higher than 0.1 mg·mL−1 (Figure 10). Nevertheless, for particles of concentration around 100 μg·mL−1, we consider that the cytotoxicity of NPs is negligible.

3.5. Observation of Particles Fluorescence in Living Cells

The spherical mSiO2-Eu(TTA)3(Phen-Si) NPs have been allowed to react with MDA-MB231 cancer cells under conditions where NPs are shown to be noncytotoxic (0.1 mg·mL−1) overnight. Images in Figure 11 show that NPs have been internalized by the cells. Indeed, a strong red fluorescence is observed in their cytoplasm with a higher intensity in the perinuclear area. The nucleus, stained in blue with DAPE, appears to be totally free of NPs as shown by time gated detection. Time gated detection collects the emission light only 100 μs after the excitation, keeping only the long-lasting luminescence of Eu3+ and removing all the background coming from the DAPE dye and self-fluorescence of the biological media [14].

4. Conclusion

We have synthesized new hybrid nanoplatforms for luminescent biolabeling based on the concept of Eu3+ complexes encapsulation inside mesoporous silica nanoparticles. Europium complexes have been selected on the basis of their capability to be exited at 365 nm which is a wavelength currently available on routine epifluorescence microscope. For Eu3+ complexes encapsulation two different routes have been used: the first route consists in grafting the transition metal complexes into the silica wall surface. The second way deals with physicochemical impregnation of the mesoporous silica NPs with the Eu complex. Then a silica shell coating will prevent any dye release. For this last protocol, the best result has been obtained using Eu-BHHCCT complex. However the best solution appears to be Eu(TTA)3(Phen-Si) complex covalently grafted inside the mesoporous silica NPs.