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Journal of Nanomaterials
Volume 2013 (2013), Article ID 958172, 8 pages
http://dx.doi.org/10.1155/2013/958172
Research Article

In Vitro Biocompatibility of Electrospun Chitosan/Collagen Scaffold

1Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
2Clinical Institute of Integrative Chinese and Western Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai 200437, China
3Department of Textile and Chemical Engineering, Changzhou Textile Garment Institute, Changzhou 213164, China

Received 14 June 2013; Revised 31 July 2013; Accepted 22 August 2013

Academic Editor: Xiaoming Li

Copyright © 2013 Peiwei Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chitosan/collagen composite nanofibrous scaffold has been greatly concerned in recent years for its favorable physicochemical properties which mimic the native extracellular matrix (ECM) both morphologically and chemically. In a previous study, we had successfully fabricated nanofibrous chitosan/collagen composite by electrospinning. In the present study, we further investigate the biocompatibility of such chitosan/collagen composite nanofiber to be used as scaffolds in vascular tissue engineering. The porcine iliac artery endothelial cells (PIECs) were employed for morphogenesis, attachment, proliferation, and phenotypic studies. Four characteristic EC markers, including two types of cell adhesion molecules, one proliferation molecule (PCNA), and one function molecule (p53), were studied by semiquantitative RT-PCR. Results showed that the chitosan/collagen composite nanofibrous scaffold could enhance the attachment, spreading, and proliferation of PIECs and preserve the EC phenotype. Our work provides profound proofs for the applicable potency of scaffolds made from chitosan/collagen composite nanofiber to be used in vascular tissue engineering.