In Vitro Biocompatibility of Electrospun Chitosan/Collagen Scaffold
Figure 5
Semiquantitative mRNA expression of PIECs growing on untreated coverslip control and electrospun chitosan/collagen nanofibrous scaffolds prepared using different ratios of chitosan and collagen. (a) A typical agarose gel showing the products of 873 bp ICAM-1, 424 bp VCAM-1, 339 bp PCNA, 430 bp p53, and 222 bp B2M (internal control) bands. (b) The mRNA levels were quantitated semiquantitatively by Quantity One software according to three independent experiments, and B2 M mRNA was used as an internal control. The densitometric values of ICAM-1, VCAM-1, PCNA, and p53 were normalized with that of B2M, respectively, and the relative amount was presented as mean ± S.E.M. *, ** versus untreated coverslip control.
