Effect of RGD Peptide-Coated TiO₂ Nanotubes on the Attachment, Proliferation, and Functionality of Bone-Related Cells

Figure 10

Bone marrow derived-macrophages under M-CSF and RANKL treatment were cultured, (a) and bone marrow cells under 1α,25(OH)₂D₃ treatment were cocultured with osteoblast (b) on uncoated (30 and 100) and RGD peptide- (P30 and P100) coated TiO₂ nanotubes. Cells were then stained with fluorescein diacetate (FDA). Cells having actin ring were counted as mature osteoclast. Bar = 500 μm.

(a)

(b)