Development of Amperometric Laccase Biosensor through Immobilizing Enzyme in Magnesium-Containing Mesoporous Silica Sieve (Mg-MCM-41)/Polyvinyl Alcohol Matrix
Figure 6
(a) The steady-state current response of Mg-MCM-41/PVA/Lac (A) and MCM-41/PVA/Lac (B) modified Au electrodes at 0.45 V to 0.05 mM catechol supported by acetate buffer solution (pH 4.8) at room temperature, respectively. (b) The calibration curves of the Mg-MCM-41/PVA/Lac/Au electrode (A) and MCM-41/PVA/Lac/Au (B) at 0.45 V (versus SCE). Inset: amperometric responses of the Mg-MCM-41/PVA/Lac/Au electrode (A) and MCM-41/PVA/Lac/Au (B) on successive additions of obtained upon the successive addition 0.5 mM catechol to stirred blank pH 4.8 acetate buffer solution at 0.45 V (versus SCE). (c) The Lineweaver-Burk curves of the Mg-MCM-41/PVA/Lac/Au electrode (A) and MCM-41/PVA/Lac/Au (B) (versus SCE).
