Titanium Oxide Nanotube Surface Topography and MicroRNA-488 Contribute to Modulating Osteogenesis
Figure 1
Induction of miR-488 involved in osteoblastic differentiation of MC3T3-E1 cells grown on culture dish. (a) The expression of miR-488 was measured using real-time PCR. Alizarin red S stain was used to monitor the mineralization of ECM (inserted photography). Cells were treated with PNA-based ASOs against miR-488 (anti-miR-488) or a scrambled PNA-based ASO (Con). (b) The expression of ALP was measured using real-time PCR. RQ of control culture was used as a control to measure fold change. (c) Cells were immunostained for F-actin with Alexa488-phalloidin. The data shown are representative of at least four independent experiments. The mean is plotted and the error bars represent 95% CI (lower/upper limit). *.
