Titanium Oxide Nanotube Surface Topography and MicroRNA-488 Contribute to Modulating Osteogenesis
Figure 2
Culture on a TiO2 nanotube surface affects osteoblastic differentiation of MC3T3-E1 cells. MC3T3-E1 cells were cultured on titanium oxide nanotube having various diameters (30, 50, 70, and 100 nm), titanium oxide (TiO2), or culture dish (Cd). (a) The expression of ALP, sp7, and Dlx5 was measured using real-time PCR and cell adhesion was assayed at day 2. RQ of cells on culture dish (Cd) was used as a control to measure fold change. (b) Cells were immunostained for F-actin with Alexa488-phalloidin at day 2. Representative data is shown (). The mean is plotted and the error bars represent 95% CI (lower/upper limit). *.
