Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles

Figure 1

Detection of a specific dengue virus target DNA sequence using AuNPs. Adsorption spectra of (A) AuNPs, and AuNPs solution in the presence of (B) PNA probe, 27-mer, (C) complex of PNA and complementary DNA, (D) mixture of PNA and noncomplementary DNA, and (E) mixture of PNA and single base mismatch DNA. Insert is the photograph image of the color changes of assay conditions (A)–(E).