Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles

Table 2

Experimental conditions for detection of specific DNA sequence, noncomplementary DNA, and single base mismatch based on the AuNPs aggregation process.

Assay	AuNPs (𝜇L)	PNA (𝜇L)	Complementary DNA (𝜇L)	Noncomplementary DNA (𝜇L)	Single base mismatch DNA (𝜇L)	Ratio (𝑉 : 𝑉)
A	160	—	—	—	—	—
B	150	10	—	—	—	—
C	140	10	10	—	—	1 : 1
D	140	10	—	10	—	1 : 1
E	140	10	—	—	10	1 : 1