About this Journal Submit a Manuscript Table of Contents
Journal of Nanomaterials
Volume 2014 (2014), Article ID 907435, 9 pages
http://dx.doi.org/10.1155/2014/907435
Research Article

The Activity of [60]Fullerene Derivatives Bearing Amine and Carboxylic Solubilizing Groups against Escherichia coli: A Comparative Study

1Department of Microbiology, Orenburg State University, Pobedy Avenue 13, Orenburg 460018, Russia
2All-Russia Research Institute of Beef Cattle Breeding, 9 Yanvarya Street 29, Orenburg 460000, Russia
3Institute of Cellular and Intracellular Symbiosis, RAS, Pionerskaya Street 11, Orenburg 460000, Russia
4Institute for Problems of Chemical Physics of RAS, Academician Semenov Avenue 1, Chernogolovka, Moscow Region 142432, Russia

Received 5 September 2013; Accepted 13 November 2013; Published 15 January 2014

Academic Editor: Myoung-Woon Moon

Copyright © 2014 Dmitry G. Deryabin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We report a comparative investigation of the antibacterial activity of two water-soluble fullerene derivatives bearing protonated amine (AF) and deprotonated carboxylic (CF) groups appended to the fullerene cage via organic linkers. The negatively charged fullerene derivative CF showed no tendency to bind to the bacterial cells and, consequently, no significant antibacterial activity. In contrast, the compound AF loaded with cationic groups showed strong and partially irreversible binding to the negatively charged Escherichia coli K12 TG1 cells and to human erythrocytes, also possessing negative zeta potential. Adsorption of AF on the bacterial surface was visualized by atomic force microscopy revealing the formation of specific clusters (AF aggregates) surrounding the bacterial cell. Incubation of E. coli K12 TG1 with AF led to a dose-dependent bactericidal effect with µM. The presence of human erythrocytes in the test medium decreased the AF antibacterial activity. Thus we reveal that the water-soluble cationic fullerene derivative AF possesses promising antibacterial activity, which might be utilized in the development of novel types of chemical disinfectants.