Impact of Silver and Iron Nanoparticle Exposure on Cholesterol Uptake by Macrophages

Figure 2

Hyperspectral profiles of 20 nm AgNPs, 110 nm AgNPs, and 20 nm Fe₃O₄ NPs in either water or cholesterol (20 μg/mL). Following a 24 h incubation in water or cholesterol (20 μg/mL) NPs were collected via centrifugation and underwent a series of washes. NPs were then loaded onto premium clean microscope slides and assessed by hyperspectral darkfield microscopy. NP spectra were created utilizing pixels with an intensity of greater than 1,000. Numbers represent the wavelength of the spectral peak; black denotes NPs in water whereas gray denotes NPs incubated with cholesterol.