Impact of Silver and Iron Nanoparticle Exposure on Cholesterol Uptake by Macrophages

Figure 7

Confocal microscopy imaging of alterations in cholesterol uptake by macrophages exposed to NPs. (a) Control macrophages cultured in serum-free media for 24 h. (b) Macrophages cultured for 24 h in serum-free media containing cholesterol (20 μg/mL). (c) Macrophages exposed for 24 h to 110 nm AgNPs (25 μg/mL) in serum-free media. (d) Macrophages exposed for 24 h to 110 nm AgNPs (25 μg/mL) in serum-free media containing cholesterol (20 μg/mL). Blue represents DAPI stained nuclei whereas green areas represent fluorescently labeled cholesterol. All images were taken at 40x magnification with the confocal and detection parameters held constant between images.

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