Frontal Cryosectioning: An Improved Protocol for Sectioning Large Areas of Fibrous Scaffolds

Figure 5

Cross-section (a) and frontal section (b) of an air impedance scaffold seeded with human dermal fibroblasts (yellow). Notice how the spatial scaffold properties, such as the macropores (arrow), are easily visible in the frontal section whereas in the cross-section no landmarks can be confidently identified. The ability to recognize scaffold features (e.g., macropores) improves our ability to correlate certain cellular events (e.g., infiltration) with changes in scaffold architectures. Left scale bar = 200 μm (for (a), cross-section image), right scale bar = 500 μm (for (b), frontal section image).

(a)

(b)