Ultra-Sensitive Colorimetric Plasmonic Sensing and Microfluidics for Biofluid Diagnostics Using Nanohole Array

Figure 6

Schematic overview of the replica molding fabrication process. (a) The nanocone master mold made on glass substrate is first cleaned and silanized for 30 min followed by ethanol and deionized water rinse. (b) 5 μL of UV-curable polymer (NOA-61) is evenly spread on the top of the nanocone master and a PET sheet is carefully, to avoid the bubble formation, put on top of the polymer to act as a substrate. The master with the polymer and PET sheet then exposed to UV-light (105 mW cm⁻²) for 60 sec. (c) The PET substrate with nanohole arrays is peeled off carefully from the master mold. (d) Then and then in order to make the structure surface plasmon active, 90 nm of metal layer of gold or silver is deposited [46].

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