Formulation and Optimization of Eudragit RS PO-Tenofovir Nanocarriers Using Box-Behnken Experimental Design

Table 1

BBD experimental parameters and levels for preparation of nanoparticles.

	Levels
	Low (−1)	Medium (0)	High (+1)
Independent variables
= ratio of a polymer to a drug (mg)	50	75	100
= SDS (%)	3	4	5
= sonication time (min)	30	45	60
Dependant variables	Constraints
= PS (nm)	Minimum
= PDI	Minimum
= EE (%)	Maximum
= zeta potential (mV)	Maximum