Research Article

Surface Epitope Coverage Affects Binding Characteristics of Bisphenol-A Functionalized Nanoparticles in a Competitive Inhibition Assay

Figure 4

Dose-response curves of the ci-ELISA under the same molar concentration of particle in an assay. Absorbance curve (a) and inhibition curve (b) of the c-ELISA conducted using the same molar concentration cysBPAv-AuNP. ● = 2.1 × 10−10 mol cysBPAv-AuNP-190 L−1 (the concentration of epitope in an assay: 4.0 × 10−8 mol L−1); □ = 2.1 × 10−10 mol cysBPA-AuNP-396 L−1 (the concentration of epitope in an assay: 8.0 × 10−8 mol L−1); ▲ = 2.1 × 10−10 mol cysBPA-AuNP-801 L−1 (the concentration of epitope in an assay: 1.7 × 10−7 mol L−1).
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(b)