Surface Epitope Coverage Affects Binding Characteristics of Bisphenol-A Functionalized Nanoparticles in a Competitive Inhibition Assay

Table 3

Assay parameters and calculated affinity constant derived from Figure 4.

Nanoparticle	190 (●)	396 (□)	801 (▲)
Particle number (mol L^−1)	2.1 × 10^−10	2.1 × 10^−10	2.1 × 10^−10
Molar concentration of surface epitope (mol L^−1)	4.0 × 10^−8	8.0 × 10^−8	1.7 × 10^−7
Slope from Figure 4(b)	0.68 ± 0.08	0.81 ± 0.10	0.36 ± 0.14
IC50 (µmol L^−1)	0.7 ± 1.2	1.8 ± 1.2	20.0 ± 15.4
Affinity constant (, µmol^−1)	1.1 ± 0.2	0.55 ± 0.08	0.34 ± 0.10
Linear detection range (µmol L^−1)	0.09–5.2	0.32–11.8	0.20–44.7

20–80% of the % curve.