Particle Size Dependent Photodynamic Anticancer Activity of Hematoporphyrin-Conjugated Fe₃O₄ Particles

Figure 8

Confocal fluorescence images of cell membrane translocation and nuclear fragmentation in PC-3 cells. (a) Confocal fluorescence images of cell membrane translocation in PC-3 cells stained with Annexin V-FITC (green) for cell membrane and PI (red) for nucleus, after irradiation for 30 min. (b) Confocal fluorescence images of nuclear fragmentation (line arrows) in PC-3 cells stained with Hoechst 33342 (blue) for nucleus and Texas-Red (red) for whole cells. Apoptosis was induced by irradiation for 30 min after treatment with 25 μg/mL of 92.7 nm Fe₃O₄@HPs particles for 2 h.

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