Flaxseed Ingestion Alters Ratio of Enterolactone Enantiomers in Human Serum

Table 2

Human serum EL concentrations (nM) before and after flaxseed ingestion measured by chromatographic and TRFIA methods.

Laboratory	I	II	III
Method	HPLC-MS/MS	HPLC-CEAD	GC-MS	HPLC-CEAD	TR-FIA
Samples^#
1W	55	33.9	NA	32.7	28.1
2 W	22.5	12.3	NA	14.9	12.1
3 W	76.4	40	NA	53.1	28.4
4 W	16.8	11.9	NA	13.9	11.2
5 W	11	6.7	NA	12.1	9
6 M	17.2	6.9	NA	12.6	5.5
7 M	85.8	52.4	NA	54	52.9
8 M	22.5	14.5	NA	15.1	14.3
9 M	22.3	9.2	NA	13.1	10.2
10 M	10	7.3	NA	29.3	8.2
11 W	1434.8	NA	NA	NA	NA
12 W	1059.3	930.9	886.2	1016.7	359.6
13W	1850.5	NA	NA	NA	NA
14W	262.2	225.7	260.3	235.7	105.2
15W	263.8	220.8	190.8	219.8	127.9
16M	160.6	110	144.4	128.3	54.4
17M	1146	NA	NA	NA	NA
18M	620.5	NA	NA	NA	NA
19M	526.3	438	376.2	421.8	201.2
20M	221.6	161.7	167.6	142.6	108.4

^#Samples 1–10 are collected from healthy Finnish volunteers having their habitual diet and samples 11–20, are from the respective individuals after 8 days of flaxseed supplementation (25 grams per day). Samples not available for analysis due to a limited sample volume are marked as NA. Samples collected from women are marked with W and those collected from men with M.