Unique Mode of Lipogenic Activation in Rat Preputial Sebocytes

Table 1

Primers for RT-PCR.

	Primer sequence
Primer	Forward (5′ to 3′)	Reverse (5′-3′)
ACAT-1	5′-ATGGCTGCCCTGGCGGTTCTA-3′	5′-CTACAGCTTCTCAATCAGCAC-3′
Add-1	5′-ATGGATTGCACATTTGAAGAC-3′	5′-TGGTACTGTGGCCAGGATGGT-3′
Adipsin	5′-ATGCACAGCTCCGTGTACCTC-3′	5′-TCAGGCCGTCACGTTAACACT-3′
aP2	5′-TGGAAACTCGTCTCCAGTGAG-3′	5′-CAAATTTCAGTCCAGGGCCTC-3′
Apo-E	5′-ATGAAGGCTCTGTGGGCCCTG-3′	5′-TCATTGATTTCTCCAGGGCAC-3′
CD36	5′-GCAGCTGCACCACATATCTAC-3′	5′-GCTGGCTTGACCAGTATGTTG-3′
DGAT-1	5′-GCGGTTTCAGCAATTACCGTG-3′	5′-ACTGGGGCATCATAGTTGAGC-3′
HSL	5′GAAACCTAGGAGACCAATTTC-3′	5′-ACCTGCAAAGACGTTGGACAG-3′
Leptin	5′-ATGACATTTCACACACGCAGT-3′	5′-CTAGAGGAGTAGGAGAAACGG -3′
Leptin-R	5′-ATGACGTGTCAGAAATTCTATG-3′	5′-GGAAGCATTGGATCCAACACT-3′
LPL	5′-ATGGAGAGCAAAGCCCTGCTC-3′	5′-CAGAGACTTGTCATGGCATTT-3′
MC5-R	5′- ATGAACTCCTCGTCTCACCTG -3′	5′- TTAATACCTGCCAAGGAGCGT -3′
Scd-1	5′-ATGCCGGCCCACATGCTCCAA-3′	5′-TCAGCTACTCTTGTGGCTCCC-3′
SR-B1	5′-ATCATGATTCTCATGGTGCCC-3′	5′-TGGCAGCTGGTGACATCAGAG-3′

acyl-CoA:cholesterol acyltransferase type 1 (ACAT-1), adipocyte determination factor (Add-1), adipocyte fatty acid binding protein (aP2), apolipoprotein E (ApoE), fatty acid translocase (CD-36), acyl-CoA:diacylglycerol acyltransferase type 1 (DGAT1), hormone sensitive lipase (HSL), leptin receptor (Leptin-R), lipoprotein lipase (LPL), melanocortin 5 receptor (MC5-R), stearoyl-CoA desaturase type 1 (Scd-1), and scavenger receptor type BI (SR-B1).