Peroxidation of n-3 Polyunsaturated Fatty Acids Inhibits the Induction of iNOS Gene Expression in Proinflammatory Cytokine-Stimulated Hepatocytes
Figure 11
Effects of peroxidized EPA/DHA on the expression of the iNOS gene antisense transcript. Cells were treated with IL-1β (1 nM) in the presence or absence of peroxidized EPA for 3 days (a) and DHA for 1.5 days (b) (each, 500 μM) for the indicated times. Total RNA was analyzed by strand-specific RT-PCR to detect the iNOS gene antisense transcript (iNOS-AST). RT(−): a negative control PCR using total RNA without RT.
