Transient Silencing of a Type IV P-Type ATPase, Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes
Figure 1
C2C12 cells were differentiated from myoblasts to myotubes as described in Section 2. Myotubes were transfected at each concentration of siRNA (SI00906220) (0, 50, 100, and 200 nM) collected at the above time points (24, 48, and 72 h). Gapdh (housekeeping gene) and Atp10c gene mRNA expression was analyzed using quantitative PCR. The percentage of knockdown was calculated at each concentration and time point based on the expression of the mock-transfected (0 nM) samples (* based on Qiagen recommendations). Data represents three independent experiments with each sample repeated in triplicate.
