Transient Silencing of a Type IV P-Type ATPase, Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes

Figure 3

(a–c): C2C12 cells were differentiated from myoblasts to myotubes as described in Section 2. Myotubes were transfected at each concentration of siRNA (SI00906220) (0 and 50 nM) and collected at the designated time point (24 h). Proteins were collected from these samples and subjected to immunoblot analysis. Data shown are representative of multiple independent experiments (𝑛=2 to 4), all analyzed in triplicate (*𝑃<0.05, ^#𝑃<0.1).

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